FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-E12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-E12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559672
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAG1451.11685495E-519.3118131
CGCACCT2551.1277734E-917.84392266
GTGCGTG4000.017.49964732
TCCGCAC2651.884473E-917.16946464
CCGCACC2651.884473E-917.16946465
TAGGTAG4150.016.8687535
CGAGGCT2501.7049388E-816.8007470
CCCTAGA3005.5479177E-1016.3345762
CGACGGG2151.0982276E-616.27874249
GTATTAG7450.015.9743691
CCTTGCG4450.015.730029
TATACTG12700.015.7098555
CTAGGAC2503.115947E-715.4011713
CAGCGTG2503.1193485E-715.399699
GTATATA23650.015.3922911
GTATAGA7900.015.0644361
CTAAGCA5150.014.9525934
GTATAAT12950.014.8659951
CACGCAG3553.783498E-1014.78843416
TATACAG13150.014.6398955
Produced by FastQC (version 0.11.5)