FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-E05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-E05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1840694
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAG750.001834159723.33177664
TAGACTG4100.016.2197515
GCCCTAG1957.835606E-616.1549631
CGCACCT3151.1077645E-915.5566366
GTATTAG8050.014.7836311
TATACCG5450.014.7708615
CCGCACC3101.5039404E-814.67643965
GCCCGCC1450.004743440514.481791516
ATCCCGG1708.174122E-414.41276166
ATAGGGG2951.302742E-714.2374983
GTATAAG8150.014.1727581
TCTATAC8200.014.0855743
GCCGTGT1500.005939652713.9990658
TAGGTAG4152.7102942E-1013.4941745
CGCTACG5450.013.48717170
GTATAGA8600.013.4311611
GTATATG10950.013.4255841
CTATACT7850.013.3759944
CATATAC12100.013.3059813
GACGCTA9900.013.08171270