FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-E03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-E03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522225
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG12300.015.9368311
TAGGTAG6000.015.7515395
TAGACAG8600.015.4666285
TAGTCAG5300.015.1883569
TCCGCAC3806.730261E-1114.73652964
CCGCACC4453.6379788E-1214.15700365
CGCACCT5200.014.13515666
GTATATA29400.013.2158391
CTAGACA7750.013.0980544
TCTAGAC6450.013.0245293
GTATAGG8250.012.7287681
TATAGGA10700.012.4311222
CTGAGCG2852.0333046E-512.2804419
GTATATG17050.012.112861
CTAGGAC2902.427186E-512.0701463
GTACTAG8200.011.9526241
GTCCGGA2356.8525976E-411.91464111
CTAATAC14500.011.8287423
GTGTATG9500.011.790861
CCTTAGG5652.0008883E-1111.7710632
Produced by FastQC (version 0.11.5)