FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-E02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-E02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2406766
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG7050.017.8737185
TACACCG5350.015.0478915
TATACGG9600.014.949075
GTATTAG10750.014.3269851
GTATATG16750.014.0013711
GTATAGA11150.013.8130111
ATACGGT8900.013.7651076
GTATATA26750.013.7396641
TATACAG15600.013.68695455
GTATAAG10600.013.208841
TATTAGG11250.013.0676762
TACGGTG7600.012.894667
TATACCG8500.012.7656915
GTGTAAG7100.012.32515051
ACGGTGT8550.012.2803748
ACCCGCG2305.719393E-412.17358819
GGTTAGG3205.0177805E-612.0324281
GTGTATA11650.012.0183451
GTCTTAG5257.094059E-1112.0011751
CTTACAC5257.094059E-1112.0009263
Produced by FastQC (version 0.11.5)