FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-D07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-D07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423435
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG5350.019.6272165
GTATTAG11100.019.5509951
CGCGCGG1854.7536014E-617.02723354
GGTAGTC5650.016.7251767
CGCGGGA1704.388652E-516.47078756
TCCTTGC15200.015.1969258
GTCCTAA5600.015.001111
AACGCGC2352.7737442E-614.89379752
CCTCCTA4950.014.8495852
CGTCCGG1951.4154744E-414.35885132
CTTCCTA8550.014.3285471
CTAGACT4403.6379788E-1214.3189464
CCTTGCG6150.014.2272279
CTACCCC3707.039489E-1014.1899464
TCCGCAC4001.5097612E-1013.99959264
TAGACAG8550.013.9188715
TCCTAAG6550.013.8941582
TACACTG8350.013.8330745
TATACTG14700.013.810265
TATACCG7200.013.6118365
Produced by FastQC (version 0.11.5)