FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-D02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-D02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2182737
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC4450.017.30321964
CCGCACC5000.016.7998565
CGCACCT4600.016.73936766
CTAATAC10750.015.9544433
GACGCGC1859.04095E-515.13500238
ACTATGC10450.015.0716378
CCTCGCG1951.4153756E-414.3588479
TAGACTG4202.1827873E-1114.1675155
GTATTAG10300.013.9338311
CCTAGCG2801.1237025E-613.74987830
GTATAGA12500.013.7217651
ACTAGGC4203.2923708E-1013.3332159
GTATAAG10400.013.1266881
TATACTG12300.013.0902155
CTATACT10850.012.9039984
GCGGGGT3002.4401543E-612.83321913
TACACAG7100.012.8176695
GTCCTAG2751.4065234E-512.72890951
AGACCGT6050.012.7280356
GGACCGT2203.9296612E-412.7280356
Produced by FastQC (version 0.11.5)