FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-C10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-C10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717366
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGA3250.023.69273256
CGCGCGG3250.023.69273254
AACGCGC3750.022.400452
TCCTTGC15250.021.1143538
CCTTGCG8950.020.334819
ACGCGCG4100.018.78082553
GCGCGGG4450.018.09021255
CTCCTAG4100.017.9278091
GACGCGC3800.017.49999238
CGGGAAC4400.017.4996758
CCCTTGC7250.017.378988
TCCTAGC5050.016.6333488
GTATCCT9600.016.4071461
GTCCTAC4350.016.0928341
CGTCCGG3803.6379788E-1215.65788732
ATCCTTG21500.015.6276117
GTATTAG11050.015.5212111
GTCCTAA6350.015.4339151
CCCCTAG2503.1191848E-715.4008431
TCCTATA12500.015.3999922
Produced by FastQC (version 0.11.5)