FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-C05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-C05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045515
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGCG1951.8282662E-819.74364523
GTATTAG9100.017.309011
GACGCGC1906.1262926E-616.57899538
TCCTTGC11900.015.8820098
CCTATAC7300.015.8231223
CGCGCAC1350.002948081115.55521910
CTTCCTA9150.014.5366251
CGGTCGG1450.004740719714.48315369
TCGGGGG1450.00474225914.48244616
CCTTGCG6300.014.4441329
TAGGTAG4750.014.0010675
ATTAGGG4607.2759576E-1213.6966953
ACGCGCA2303.7328646E-513.6956939
TATTAGG9550.013.5612412
CGGCCGG1550.007375674313.54875768
TAGTCAG4659.094947E-1213.5480949
TAGAGTG5550.013.2442515
CTAATAC9550.013.1947213
GTACTAG6650.013.1588971
TAGATAG7250.013.0354755
Produced by FastQC (version 0.11.5)