FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-B09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-B09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040824
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT3700.023.64950266
CCGCACC4100.021.34118865
TCCGCAC3850.020.9088264
GTCGGGG1150.001008470318.26063315
TCTATAC10900.017.3409253
CTATACT9100.016.9245174
TACACCG3301.2551027E-1015.9104445
TATACAG10300.015.6323995
CGGTCGG1350.00294790815.55535313
CTAATAC10000.015.4013113
ACTATGC8450.015.3252468
CTATGCA6500.015.0767289
CTATACA10350.014.54234
TAATACT16200.014.4765414
TATACTG12050.014.2335755
CCGATCG1500.005937827313.9998199
CGGGGGC1750.001018520913.99981817
CCTATAC5800.013.880493
GTCCTAG3051.9545587E-713.7723381
CGGGTCC2303.7334095E-513.69547625
Produced by FastQC (version 0.11.5)