FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-A02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-A02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291817
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC4550.021.5382865
TCCGCAC4700.020.8508964
CGCACCT5100.018.52925566
ACTATGC11450.017.4234358
CTAATAC10200.016.815053
GTATTAG9350.016.0989321
TATACGG6100.016.066495
GTATATA23450.014.7785751
TACACTG7150.014.1966145
CTATGCA10150.014.1378129
ACTACAC6000.014.0011043
GCGTGAC2001.7550543E-413.99988313
ACCGCGC1500.00593794313.9998828
CGCTCGT3051.958324E-713.77037634
GTCCTAC2607.888906E-613.4637751
TATACAG14350.013.4153995
TACTATG13050.013.4098487
TAGGTAG5051.8189894E-1213.1690675
TAATACT17650.013.0885654
TAGGACT5103.6379788E-1213.0399584
Produced by FastQC (version 0.11.5)