FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP2-H05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP2-H05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260664
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG9500.017.6864131
TCCGCAC4150.016.86658564
CGCGTGA1250.001763403116.7998631
GTATAGA11000.015.5928511
TAGGTAG6250.015.1215485
CCTAGAC4201.8189894E-1215.0015353
GTATATA26600.014.8702731
CCGTGCA3604.674803E-1014.5825689
GGTAGTC6000.014.5825687
TAGTCAG5350.014.3917699
CCGCACC5000.013.99926565
ATACGGT9400.013.7780076
ACGGTGT7500.013.5326238
CGGTCGG2102.6549003E-413.33263313
TATACGG9000.013.2235765
CGCACCT4304.765752E-1013.02372566
CCTAGCG3254.241556E-712.92297130
TACGGTG7350.012.8564697
ATACAGT12350.012.7543426
TATTAGG13800.012.6824582
Produced by FastQC (version 0.11.5)