FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP2-G12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP2-G12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001089
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTG1154.1215404E-521.3043029
TAGGTAG4800.020.4171355
GTATTAG8950.016.8160271
TACACAG6300.016.6670485
GTGTAAG5300.015.8494191
CTTACAC4550.015.3849673
CCGCACC3101.502849E-814.67738765
GTATAGA10550.014.5974911
CCGCGCG1450.004741598414.4827279
ACCACGC3454.0054147E-914.2028688
CAGTGCG2505.268106E-613.99996959
TACACCG4051.8371793E-1013.82747655
CGCACCT3801.0495569E-913.8157666
TATTAGG8900.013.76435852
CTAATAC8650.013.757543
TTAGGTA7950.013.6481114
GTCGCGG1550.00737703413.54835836
TATACTG12700.013.5042465
CATATAC13700.013.2849753
GTGTTAG7150.013.2170861
Produced by FastQC (version 0.11.5)