FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP2-D07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP2-D07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779186
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGC1107.4800936E-419.09061621
GCGCGTG1200.001341596617.49973331
TCCGCAC3203.6379788E-1217.4992464
CCGCACC3600.017.4992465
GCGCTCG1854.7534068E-617.02676833
TAGGTAG3753.6379788E-1215.8682095
CCTAGCG2452.4805922E-715.71404630
CGCACCT3704.3655746E-1115.13575566
TATACTG13000.015.0783885
GCTCGCC1400.003755378514.99977222
TATACCG5500.014.6377875
GCGACTC2656.035407E-714.5280834
CTCGCCC1450.00474173714.48253723
GTATTAG9500.014.0025411
CACCTAG3252.7994247E-813.999392528
TTATACT13800.013.950634
CTAATAC9550.013.9284463
CCTAGGG1850.001547342613.2449033
GTGCGCG1600.00910123713.1244319
TAGACAG5350.013.0853845
Produced by FastQC (version 0.11.5)