FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP2-C12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP2-C12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022441
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG3950.026.58244754
CGCGGGA4250.025.52957356
AACGCGC5350.021.58892452
TCCTTGC13550.021.4385418
GCGCGGG5950.018.82364855
CCCCTAG2659.458745E-1118.4919071
CCTTGCG9450.017.7773049
CGGGAAC6450.016.27863558
CCTACAC6400.015.8600023
TCCTCAC11000.015.5904938
TAGGTAG5600.015.0005925
ACGCGCG7000.015.00009553
TACGTCC5000.014.700584
CCGAGGC13850.014.65737570
GTCCTAA6250.014.5610571
TCCTAGG2656.035716E-714.5288753
CTAATAC13750.014.2551093
ATCCTTG20100.014.1043347
CTCCTAC7950.014.0890721
CGGGTCG3001.6015838E-713.99962618
Produced by FastQC (version 0.11.5)