FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP2-C10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP2-C10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125230
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCG1906.1262144E-616.5790831
CGTCCGG1957.84129E-616.15397832
CGGGTCC1807.154924E-515.55568125
TCCTAGG2755.5155397E-815.2728492
TCTAGAC5100.015.0981623
CTCCTAA5600.014.3751151
GTCCTAC2707.454546E-714.2593751
GTCCTAA4303.274181E-1113.8373211
GGACCGT3051.9577055E-713.7706026
GCCCTAG2052.1635607E-413.6586471
GTATTAG9000.013.6112211
CCCTACG1800.001259810513.611221
ACCGCGC1550.007377882513.5481788
CTCCTAT6750.013.481591
CCTATAC8050.013.4783693
TCGCGGC2354.5690656E-513.40436347
CCTCCTA2901.6673857E-613.2759691
CTTACAC6100.013.1968273
CCCCTAT3456.123082E-813.1885121
TCCTATA10400.013.1251062
Produced by FastQC (version 0.11.5)