FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP2-B07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP2-B07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212792
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG6200.015.8073695
TAGGTAG5100.015.0989155
GTATTAG9550.015.0273911
CTAATAC10600.014.8593543
TATACAG13850.014.6578915
TAGGACC4550.014.6162344
GTCCTAG2707.4488344E-714.2604091
CGCACCT4202.1827873E-1114.1671766
GTATAGC6250.014.001131
GTATAAG10900.013.80845451
TACGGTG5850.013.76055057
CTAGTAC7400.013.7170123
TCCGCAC4354.0017767E-1113.67802964
CTGTGCG3152.9009425E-713.3332059
CATATAC14750.013.2889083
TCCTAGA5600.013.126062
CCTACAC4002.2591848E-913.1257623
ACCGTGC2405.5685276E-513.1248738
GTATAGA11300.013.00991
GTATATA23950.013.0073121
Produced by FastQC (version 0.11.5)