FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP2-B04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP2-B04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533702
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG6550.018.7028755
GTATTAG12650.017.4317191
GCTCGCG2903.43789E-1016.89608261
TCTATAC12700.014.6070443
CCGCACC4850.014.43258665
TCCGCAC4505.456968E-1213.9996164
TATACTG16450.013.830225
TATACAG20000.013.8254335
GTGTATA11200.013.7507021
AGCTCGC3607.2923285E-913.6107360
CGCACCT4901.8189894E-1213.57185366
GTATATA32400.013.5037751
TAGACTG6350.013.228765
GGTAGTC8250.013.1511487
CATATAC21300.013.1462113
CTCGCGT4002.2628228E-913.12463462
ACTGCGC3507.3965566E-812.9996388
CTATACA12450.012.9321324
TATACCG7400.012.7706715
GTATAGA13450.012.7515791
Produced by FastQC (version 0.11.5)