FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP2-B01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP2-B01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685268
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATCG2053.2456228E-818.7798359
TAGGTAG3300.018.0328875
GTATTAG7350.015.7170041
GCGCTCG1605.1640964E-415.31242233
GTCCGGC1859.040999E-515.13460911
CTAATAC7250.014.9676623
GTATAGA8650.014.5689941
CGCGCAT1750.001018330913.99992840
CGGCCCG1750.001018559313.99951314
CCGGCCC2001.7550116E-413.99951313
CCCGCCC1750.001018559313.99951317
GTATATA19450.013.8584381
TCTATAC7850.013.8236363
CTGCTCG2556.466167E-613.7250139
GGACAGC2901.664268E-613.2777646
GCCCGCC1850.001549149713.24278416
TCCGCAC2659.618228E-613.207087564
CCTAATA8550.013.1012932
GTATAAA12200.012.9120691
TAATACT14050.012.7064474
Produced by FastQC (version 0.11.5)