FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641831
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTCG1852.2736458E-718.9187632
TCCGCAC3300.018.03015364
CTAATAC8200.017.073553
GTATAGA8500.016.4714571
CGCACCT3651.8189894E-1216.30173366
CTAGGAC2803.910827E-916.250363
TACACCG2602.7659553E-816.1542055
ACCGTGT1901.13446295E-414.736728
TAGGACT3352.6429916E-914.6271914
TCTAGGA4550.014.6157082
CGCGAGG1450.00474131114.48263813
CCGCACC3901.0186341E-1014.35885665
TATACGG6200.014.1132165
TCGGGGG1500.005936990513.99988434
GTATATG11150.013.8123871
GTCCTAG2303.730235E-513.6963741
TATAGGG5650.013.6286212
TACACGG1550.007375104413.5486885
CTACACA4404.7293724E-1113.5230264
TCTATAC7000.013.50033
Produced by FastQC (version 0.11.5)