FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-H08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-H08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9612961
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC17050.015.80594464
CCGCACC17900.015.25090865
TATACGG33950.014.330635
TAGGTAG20250.014.1735645
CGCACCT18500.013.81144566
ACCGTGC10800.013.6106758
CTAATAC44350.013.1799313
ACTATGC46750.013.1760478
GTATTAG41350.013.1208591
TATACAG62050.012.9176635
GTATAGA43400.012.7430511
GTATATA93450.012.6602841
TATACTG53200.012.5664325
TCTAGAC22950.012.5060863
TATACCG29900.012.1745365
TCTATAC44050.012.1572623
CATATAC60750.012.1570013
CTATGCA39650.012.0929289
ATACGGT35950.012.072946
TAATACT84400.012.0266814
Produced by FastQC (version 0.11.5)