FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-H03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-H03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCCTTTCTTTGCGGAAAATAATCAAAAAATGTACCAAAAAATTGCCT11.6129032258064515No Hit
CCTGCATCCATTGCTTATCATTTAATTCATTGCTATGAGGAGCTTTACCG11.6129032258064515No Hit
CTCTCAGTGAGGCCAATCGTAGCAGGAATAACATGATTACTGCTCGAATA11.6129032258064515No Hit
GGCAAGCAACGAACCAGAAAATTTAGAAGCCCATGTTACAAATGGGATAC11.6129032258064515No Hit
GTTAATACAGTTTTTTCAAGAATTGCAAGATCCCATGGTGATAAGCTGCT11.6129032258064515No Hit
GTTACAAAGACAGAAACTTGACAAATCAAGGTCAACTGGATCGTTGCTCG11.6129032258064515No Hit
GATTCATTATGTGAAAAATGGCAGTCTGAAGCGGAAGATCAAACTGAGGC11.6129032258064515No Hit
AAATGATAGTAAAGGCAAATTGAATTAGATGAGCAAAAGTCTTCCATATA11.6129032258064515No Hit
CTACATGGCCAATTATCAAAAACCCAGCCCAATTTTTTACTGCTTATGTT11.6129032258064515No Hit
CTACCCACAGACCCAGAAAATGTCGCGGACTTTGACTTGGGCGATTTGCA11.6129032258064515No Hit
TCATAAAACTGATACGAGCTTCTGCTATCCTGAGGGAAACTTCGGCAGGA11.6129032258064515No Hit
ATTAATACATATTATCATTGACCTATAGTTTTCAAGGCTGCAGCTGCTGA11.6129032258064515No Hit
CAATTTACGCTATGATCTGTAAGCTAAATGATCACTGTGATGAACTGTAA11.6129032258064515No Hit
TTCCCAATCGTTTCCAGTTATCCTATAAAGTGAACTGGATCTATTAATTA11.6129032258064515No Hit
CCATTACGATGGTTAAGGCAGTTTACGACGAATTGCGGAGTCAAGTTTTA11.6129032258064515No Hit
TATGAACGAACACTAGCTTACAAATGTGGAAACGGGATCCTTCAGAGGTA11.6129032258064515No Hit
ATACAAAAGGTGTGCATGCGGCGGCTCCATCAACCAATGACCATGGTGTG11.6129032258064515No Hit
TGATTCAAGTTGGTTGATAAGACTGAATCAGTCGAAAAAATTTCCATCGG11.6129032258064515No Hit
CCTCTATTATCTTGCATAGCATCTATTGATATGTTCTCGCTATTTACCAC11.6129032258064515No Hit
TAATAACACCTACATTTCATAATAGTACTCTTGGTGATATATATGAACCA11.6129032258064515No Hit
GTCAAGGACACCGTCAAGCAGGACACAAAGGCAAATATTACCGATGTTTG11.6129032258064515No Hit
TCTCACAACGATGCCTTGTTTGCTGCTGTCAAAGGTGCACCTGAAAAGAT11.6129032258064515No Hit
GTATTGAGACCATCAGACTAGCAAACGTAAGTCAAAAGCTTGTTTTATAG11.6129032258064515No Hit
ACACAACCAAGGGCAAATACGTGGTTAGTGGTGTAAAAACTGAAGGGATT11.6129032258064515No Hit
AGATATAGGCATGTATATCTTCCAAGTAATATACGTGTTTGGGACGAACA11.6129032258064515No Hit
GTTCAGTAAGTGAGCCCAAATCAGTAAATCTTATGATCCACTTTAACATC11.6129032258064515No Hit
ACTAGAAGTGGGTGTTTCTAGTAGCCTGCAAAAAGCGTCGTTCTAAAGAA11.6129032258064515No Hit
TCGAGGCAAATGGTCTGTGAATGAAGCTCACTTACGCTGCAATAAGCAGC11.6129032258064515No Hit
CTTTGAGCGATTCGTTTAACAACAGCTCTTCTTCTACGAAGTATGATGTA11.6129032258064515No Hit
ATTATAAGAAATACAAAACGTTAGCATTTGCATTTGTTGGACATGTACTG11.6129032258064515No Hit
GGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGCGCA11.6129032258064515No Hit
AACAAAGCCACAAAATTGGGACAGAGTTGTCCCTAAATTCAATACTGGTA11.6129032258064515No Hit
GAGGATGCTCAATGGATAATAGTAAGAAATAAGACGGAAACTCCTTTTTG11.6129032258064515No Hit
TCCTAGAATCGAACCAGGGTTTCATCGGCCACAACGATGTGTACTACCAA11.6129032258064515No Hit
CCGGGGGCGAGTCGAACGCCCGATCTCAAGATTTCGTAGTGATAAATTAC11.6129032258064515No Hit
GAAAATATACATTTATCACGGTGCTTCCAGAACCACGCATATCAAAGATT11.6129032258064515No Hit
ATAAAAAAGGACCTCATCGTCAGGGTGCTTCGTTTTTCTAATATGCGAGT11.6129032258064515No Hit
GAACAACAGTCTCTCCCCACCCCTTTCCAAAAAGGGGAATGAAATACGAG11.6129032258064515No Hit
CCCTTGGGTTGCTTGCACAGGAAGAACACGTATCTGTGATAACCGGAGTT11.6129032258064515No Hit
AAGATATACTGCGTTGGAACCAGTAAAATAAAGCACGCAATACAATCAAC11.6129032258064515No Hit
TATATACGTTGAAAAGAAAATAACAAATCCGCCAGATGATGTTGTTAATC11.6129032258064515No Hit
AACATTAACATTAGTTCTTTTGCTAAAGTTTCTCCAATATCTGGAATATC11.6129032258064515No Hit
TAGCAAGATCATCGTAGATCCCCACGTATAAATCAGTAACGCCATCGTAA11.6129032258064515No Hit
TCACCGAGTTGCGTCGTAGTGGCAGAACCGCCATTGACCAGTTCGTTACC11.6129032258064515No Hit
GACGGGAAACGGTGCTTTCTGGTAGATATGGCCGCAACCGATACTTTAAC11.6129032258064515No Hit
AAACTCCACTGCCTGTGTTGGACTTCATACGAGATTTTTTTCACCGTTGA11.6129032258064515No Hit
AGACAAGGTAGTTGTCGAGCCCACAGGTACATGCAGAGACCGGTATCGTT11.6129032258064515No Hit
ACATAACGCCTCTTTAGATAAATTATCATAACCATTTTTACACTAAATAA11.6129032258064515No Hit
ACTCCGATGCCATTAATCTATCATAAAGTCTGGCGGCGTATTCCTTGCAC11.6129032258064515No Hit
CACTAGATCTTACGTTAGTACTGCTGAAAATGGCAATAATTGTTAAAGCA11.6129032258064515No Hit
TTGTACTATATTTGGGGCCCCTTTCAATTTCTTCATTACCTCCACTTCAT11.6129032258064515No Hit
TAACTAAACATACTAACCCCATAGGTAATACTCAAAAAGCAATGTTATTA11.6129032258064515No Hit
GACGGGAAACGGTGCTTTCTGGTAGATATGGCCGCAACCGATAGTTTAAC11.6129032258064515No Hit
CTGAAGACGCACTTTCAAAAAACCAAAAACGCACCGGACTGTAACGAGCT11.6129032258064515No Hit
TACCAGGGTTGATATGGAATCTGTCTGGCGATTTGGTTTGTTTTAGTTGG11.6129032258064515No Hit
AAAAACTTTCCACCCAATATCTCCTTTAATCTTAGTTATAATACAAAATG11.6129032258064515No Hit
ATCCTGGAGTAACTTCGTTTAATCTGCCTCGTTTTTTTCTCTAAATAGTT11.6129032258064515No Hit
TGTCTACGCCTAATGGATCGAGGTCTGCAATTTCTGGTTTTTTCTTCTTC11.6129032258064515No Hit
GACCTTATTCACCCAAAAACATCGGGGAAACGGTAGTAATATGGAAGCTG11.6129032258064515No Hit
CAATTACTATTGCGGTAACATTCATAAGTAGGGTAAAACTAACCTGTCTC11.6129032258064515No Hit
GTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTC11.6129032258064515No Hit
GTTAAGGAGCCCTCGTTTCCCTCTAGGAGCGCTTTTTCACCTATTTTATC11.6129032258064515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGA50.070.053
CAAGATG50.070.064
TTTGACT50.070.031
TTTGACG50.070.055
GTTTGAC50.070.054
TTGCAAT50.070.046
TCAAGAT50.070.063
CCCAGAA50.070.012
TGACTTG50.070.033
ACAGACC50.070.07
GCGATTT50.070.041
TGCAATC50.070.047
ACTTGGG50.070.035
GGACTTT50.070.027
ATGACGC50.070.068
GTCGCGG50.070.022
GAAAATG50.070.016
TCGCGGA50.070.023
GGCGATT50.070.040
CCAGAAA50.070.013
Produced by FastQC (version 0.11.5)