FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-G07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-G07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817902
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT5950.020.00052366
GTATTAG12450.019.9635491
CTATGCA10500.019.6657839
CCGCACC6450.018.44878465
GTCCTAC3650.018.2225651
GTATAGA12950.017.3005141
CCTAATA14450.016.9572662
CCTAGCG4350.016.89639330
CTAATAC14600.016.7830473
ACTATGC12250.016.2849858
TAGGTAG7150.016.1559895
TCCGCAC7200.016.04094764
GTATAGG8600.015.8750431
TAGTCAG6300.015.5548579
TATTAGG13650.015.1302112
GGTAGTC6950.014.6046987
ACATAGG6950.014.60417931
TATACAG16850.014.3342455
TACACCG6150.014.2295285
TATACGG10650.014.1333295