FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-F11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-F11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4305204
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC7200.017.4993864
CCGCACC7750.016.70908565
TAGGTAG10900.016.376335
CGCACCT8350.016.34824666
GTATTAG17950.015.0144431
CTAATAC20200.014.9011563
GTATAGA22300.014.753871
TATACAG27600.013.949435
GTATATA45950.013.55868151
CATATAC25550.013.4248083
GGTAGTC10800.013.2867237
TATACGG15600.013.2373275
TATTAGG20700.013.1888592
TATACTG24950.013.1865195
GTATAAG19100.012.8276651
GTGTAGG7750.012.1939681
ACCGCGC3458.754014E-712.1734838
TAGATAG16900.012.0119685
TCTATAC19550.011.9950183
GGCGTCG5853.6379788E-1211.96580632
Produced by FastQC (version 0.11.5)