FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-E11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-E11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2903061
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC5600.021.24970664
CCGCACC6200.020.32230265
CGCACCT6800.018.52947666
CTAATAC13950.016.812123
GTATAGA14850.015.5580221
GTATTAG13000.015.3485871
ACCGCGC1901.1349923E-414.7366398
TATACCG8500.014.4133045
CTAGGGG2202.4537972E-514.3199583
TAGACAG8100.014.2607825
TATACGG11000.013.683285
ACTATGC15950.013.3853958
TGCGGGG5600.013.12481912
AGACCGC4558.367351E-1113.078326
TAGGTAG6700.013.0610975
TAATACT24000.012.9805524
CGCGCAC1900.001893788112.89455910
CCTACAC6250.012.8815973
GTCTAGA7450.012.6865741
TACTATG19050.012.6776467
Produced by FastQC (version 0.11.5)