FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-E10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-E10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3669781
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG14650.018.3962691
TAGGTAG9400.016.7554235
GTATAGA16300.016.534071
TAGTCAG8850.015.4234049
CCGCACC7550.015.29769265
TCCGCAC7650.015.09772264
CTAATAC17200.015.0582333
TAGGACT6750.014.5186094
TATACAG24050.014.2620425
CGCGGCC3001.6017839E-713.99970613
CGCACCT8300.013.91650966
TAGGGGT5100.013.7255754
GGTAGTC9800.013.5715147
GTATATA35700.013.5296811
TATTAGG17200.013.4303162
ACTATGC16450.013.4039758
TTAGGTA15500.013.3226644
TCTATAC16850.013.2938523
ACCGCGC3002.4417768E-612.8330648
TATACCG11600.012.6724925
Produced by FastQC (version 0.11.5)