FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-E07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-E07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080329
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG21700.015.1619855
GTATTAG12700.014.8830571
GTATAGA14650.014.3355951
CGCACCT5900.014.23840466
CTATACT15200.014.0466974
CCTAGCG5000.013.99996130
CTAATAC13800.013.6962813
CCGTGCG1550.007379837313.5479099
CTAGGGC3901.549779E-913.4621583
GTGTATA12800.012.852571
TAGGTAG7650.012.8110465
TATTAGG14300.012.7280652
CTATGCA11550.012.7268249
CTTACAC9100.012.692893
TAATACT23200.012.67299654
GTATATA32700.012.63095951
ACTATGC13750.012.4722878
TAGGGGT4601.3860699E-912.1744734
CATATAC21600.012.1533373
CCGGTCG2902.4309049E-512.0685410
Produced by FastQC (version 0.11.5)