FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-E03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-E03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537919
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG11650.015.323321
TCTATAC12200.014.9191333
ACAGTGC7100.014.7880758
TCCTATA11150.014.7544522
CAGTGCG2858.5539796E-814.7361889
CCTACAC4301.8189894E-1214.6522443
TAGACTG6250.014.5610735
CGCACCT4950.014.14162166
GTATATA27500.014.1285931
GTCCTAC3758.60382E-1014.00130751
CTAATAC12500.013.7210113
TACGGTG9450.013.7033637
TACACAG8750.013.6010045
CCTTATA11400.013.5097692
TAGGTAG6050.013.3067675
TATACTG16600.013.2841125
CCCTACA5300.013.2085222
CTTAGAC7250.013.0354443
ATCCTAC10600.012.8785621
CTATACA13600.012.8685954
Produced by FastQC (version 0.11.5)