FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-E02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-E02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1938665
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG7450.019.732431
CGTCGCG1551.9862995E-518.0639365
CGCACCT3500.018.00034566
CTAATAC8200.016.646233
TAGTCAG4000.016.624469
ACCGCTC2653.5000994E-815.8485438
TATTAGG8650.015.7802432
TCCGCAC4000.015.74948964
GTCGCGA1807.153728E-515.55585466
ATACGGT6300.015.5554526
TAGGTAG5950.015.2940175
ACTATGC8650.014.9706138
CCTAATA9850.014.9237592
CCTCGCG1656.5245957E-414.8480039
TATACGG7550.014.8343385
TATACTG13000.014.8075955
CATATAC12550.014.7807793
ACTGTGC5000.014.6995228
GTATAGA8450.014.4976931
TACGGTG6550.014.4270127
Produced by FastQC (version 0.11.5)