FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-D07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-D07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3356851
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG10550.015.9248085
GTATTAG12150.015.8449631
CTAATAC15150.015.7101993
TACACCG6650.014.7374325
GTATAGG11650.014.4218231
TCCGCAC5900.014.23722164
TAATACT24150.013.7686664
TAGACTG6400.013.6724215
TATACGG11850.013.5870415
TATACAG17250.013.1889335
TATAGGG10250.012.9763212
TCTATAC14200.012.8174143
CGCACCT6750.012.444386566
CTACCCC5651.8189894E-1212.3898764
GTATATA31950.012.37976551
ACCGTGC3701.5271507E-712.297248
ACTGCCC6300.012.2221658
TAGGTAG7500.012.1338195
TAGGACA7600.011.9741624
CTAGTAC11300.011.7703833
Produced by FastQC (version 0.11.5)