FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-D06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-D06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804066
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG2004.3655746E-1122.75074670
CTCCTAG850.003790432420.5876311
GATCGGG2051.4515535E-920.48847669
CTTAAGG1107.4759213E-419.0903513
AGGATCG4750.018.42165870
AGGTAGC1200.001340856817.4994877
GCGCACT1200.001340856817.49948711
AGTGGTC1452.4387213E-416.8960558
CCTAAGT1250.001762317616.7995073
CCTCGCA1503.158365E-416.3328539
GAGGATC5700.015.96543769
GCCCACC1350.002944929515.55606770
GGATCGG2704.389767E-815.55606768
CATAGGA1807.148368E-515.55512
ATGTAGG1350.002946150515.55509951
CTATACA2302.2146087E-615.2169454
CGTGGGC1400.003753346414.999568
TCCTAAG1400.003753346414.999562
TGCGCGT1400.003753346414.9995635
CGGTCAG1400.003753346414.9995615
Produced by FastQC (version 0.11.5)