FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-D02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-D02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061268
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG4550.030.76971254
CGCGGGA4600.028.91349456
CCGGAGC5600.028.12475640
CGCACCG5650.027.87586636
CGGGCCG6050.026.03283322
GCGCACC6500.024.76901435
ACGGGCC6250.024.07979221
TCACGGG6700.024.02964419
AGCGCAC6600.023.86343234
AACGCGC6050.021.98381452
CACGGGC6850.021.97061320
CTCCTTA8050.021.7399961
GCGCGGG6300.021.66700455
CGTCCGG6400.021.3279432
ACCCAGT7600.020.7245081
TCCTCAC8450.020.7098798
CGGGAAC6600.020.6816458
CGTCACA7850.020.50937727
TCCTGTG8900.020.4492597
GGCCGTC7750.020.32240524
Produced by FastQC (version 0.11.5)