FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-C11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-C11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2302990
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC9000.016.334243
CCGCTCC2302.218514E-615.2172469
CGCACCT4051.0913936E-1114.69153666
GTATTAG9500.014.3695311
TATACGG8500.014.0007785
GGACCGC2001.754108E-414.0007766
TACACAG8350.013.8331025
TCCCTAG4102.237357E-1013.6595921
CTTACAC6200.013.5491383
ATACGGT9500.013.2638956
TCCGCAC4506.91216E-1113.22209564
GCGCGGT2405.5687204E-513.12487311
AGCGCGT2153.2383073E-413.023138
CATATAC14450.012.8380833
GTATAGG7100.012.8178911
ACAGCTC7100.012.8167788
GTGTATA9650.012.6952811
TAGACAG8300.012.6513055
TATACAG13600.012.6109945
CTACACA7800.012.56484
Produced by FastQC (version 0.11.5)