FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-B11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-B11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995631
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG9000.018.278531
TAGGTAG5900.017.2036675
TATACAG13450.016.6545435
GTATAGA11850.015.9500231
GTATATA24700.015.7293911
GTACTAG7050.014.894231
CCGCACC3604.656613E-1014.58320265
GGACCGT2454.272839E-614.2859446
TAGACTG3951.2369128E-1014.17744355
TGGGGCG2052.1633087E-413.6587565
TCCGCAC3851.2769306E-913.63624264
GGACCAT6200.013.5486056
CTAGGAC3102.386314E-713.5486053
GCGGGGT3102.3870416E-713.54826513
TGCGGGG2851.371327E-613.50865212
TATTAGG11450.013.4499972
TATACCG6300.013.3335485
TCGTGCG2102.6536782E-413.3332149
GTCCTAG2659.61281E-613.2080911
CCTACAC4002.2610038E-913.1252123
Produced by FastQC (version 0.11.5)