FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-B03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-B03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2781127
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC4800.017.5016273
CTAATAC11600.015.99286653
GTATTAG11350.015.72891
TAGGTAG6650.015.2643025
CGCACCT5650.014.24808866
CCGCACC6400.014.21828365
TCCGCAC5700.014.12234264
GCGCGAA2001.7556308E-413.9995399
GTATATG16200.013.6128651
GTGTAAG6450.013.5676411
TACACCG5500.013.3646385
TTAGGTA11000.013.04643254
GCGCACT4355.7480065E-1012.8731411
CCTAATA14200.012.8183242
GTGTAGG4952.5465852E-1112.7289131
CCTGGAC5003.092282E-1112.6011723
ACCGCGC2508.165534E-512.5995868
GGTAGTC7050.012.4111637
GCGCGGA2000.002783809312.24959717
TATACCG8350.012.15665
Produced by FastQC (version 0.11.5)