FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-A04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-A04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306228
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGG4600.016.7406442
CGGGGCT2009.963693E-615.7514244
TATACAG13250.014.5296155
TATACGG7500.014.4679765
TCTATAC9300.014.3023693
GTATAGG7300.013.9059711
ACAGTCC4102.237357E-1013.6579948
TACAGCG4404.7293724E-1113.5224857
CTATACA11050.013.3043714
TATATAC23900.013.18110753
GTATATA21500.013.0249991
TAGGTAG5501.8189894E-1212.7284235
GTATAGA10900.012.5246111
ACCCGCG2254.7536351E-412.44395163
ACTATGC11300.012.3888898
ACCTAAT12850.012.258451
TCCGCAC5155.0931703E-1112.23252564
CCTAATA12900.012.2104062
CGCTACG6600.012.19780870
GTCTTAG4902.8921932E-1012.1444821
Produced by FastQC (version 0.11.5)