FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-A03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-A03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558105
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC3950.015.94931464
GTATTAG10600.015.5203331
ACGGTGT8400.014.999958
TATACGG10850.014.8389515
CATATAC18000.014.7780183
GTATAGG7600.014.7382331
TACGGTG9150.014.535477
GTACTAG9000.014.3902471
CGCGCAT2707.45722E-714.25921240
GACGCGC2707.45722E-714.25921238
CTAATAC11700.014.0600573
CTAGGGT3303.4204277E-813.7881024
TATACAG17700.013.2488015
CCGCACC4506.91216E-1113.22217865
TATACCG7450.013.1545755
CGACGGG2952.0210646E-613.05080349
CTAGGCA11600.012.97409370
GACTAGG16400.012.80483570
TATATAC34400.012.7182293
GTATATA29750.012.7070821
Produced by FastQC (version 0.11.5)