FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP1-A02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP1-A02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4663851
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC17700.016.2154623
GTATTAG18900.015.0010371
TACACAG15750.014.4451335
CCTAATA21100.013.93446352
TAGGTAG9800.013.92923455
CGCACCT9550.013.92691766
TATACCG12650.013.5579585
TCCGCAC9350.013.47556664
ACTATGC17550.012.9626078
CCTACAC8650.012.9485943
TATACAG24750.012.86935
CACGAGC8000.012.68769670
CCGCACC10650.012.48792165
CCTATAC13750.012.4733213
TCTATAC19800.012.3743273
TAATACT33450.012.3473754
CCTAGAC9950.012.3121443
TATACTG27150.012.247365
ACTCCGC8600.012.2089678
TATATAC43550.012.0556843
Produced by FastQC (version 0.11.5)