FastQCFastQC Report
Sat 10 Aug 2019
H7HLTBGXC_n02_ba181.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HLTBGXC_n02_ba181.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485087517
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50381231.0386008345788869No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAACAAGCGAGATAGGAA11732820.2418701695842649No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG11652150.24020717069905556No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA8913330.1837468433556908No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG8590150.17708454039644972No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA7752790.15982250064785733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT30192250.081.9961241
AGCAGTG34503550.071.6088262
GGTATCA36105800.068.229948
GTGGTAT36164600.068.083186
GTATCAA36846400.066.9140559
TGGTATC37500800.065.605827
AGTGGTA37813250.065.2020655
GCAGTGG38989100.063.3368263
CAGTGGT39892750.061.859594
ATCAACG35729150.038.69294710-11
CAACGCA36565450.037.85339712-13
ACGCAGA36890200.037.5198114-15
AGTACAT36781150.037.21748420-21
AGAGTAC37910950.036.2653218-19
TACATGG37085750.036.14518722-23
GCAGAGT38559900.035.74384316-17
TCAACGC35980700.034.3082212-13
CATGGGG24153700.034.1148624-25
TATCAAC36319650.034.08274510-11
CGCAGAG36484050.033.837416-17
Produced by FastQC (version 0.11.5)