FastQCFastQC Report
Sat 10 Aug 2019
H7HLTBGXC_n01_ba181.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HLTBGXC_n01_ba181.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485087517
Sequences flagged as poor quality0
Sequence length27
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAGG1495200.018.6245441
CAATGGA1707400.018.5168298
GATCGCG1737400.018.5142771
GACTAAC1790750.018.3234441
CCTTACG2201550.018.1812461
GACCTGG1449700.017.794731
TGGGCGT1314800.017.7861861
GGGACCT1152400.017.7860551
GGGAATG1293300.017.7781051
CGTGAGC937350.017.7696171
GAATGAA1697450.017.7216641
CATGGCG1634900.017.7205681
GCTGCAG1516900.017.657331
AGATATG1945650.017.6373278
GGCTCGA1181800.017.5933441
AAGACCT1617000.017.5730931
GACACGC1789150.017.554291
TCGAGGG758400.017.5313762
ATGCGAT1342000.017.5243151
GATGAAA1332950.017.5172081
Produced by FastQC (version 0.11.5)