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        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2023-12-11, 14:16 based on data in: /scratch/gencore/logs/html/H7GWJBGXV/merged


        General Statistics

        Showing 330/330 rows and 4/5 columns.
        Sample Name% Dups% GCLengthM Seqs
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_A10a
        0.0%
        48%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_A11a
        0.0%
        47%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_A1a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_A2a
        0.0%
        46%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_A3a
        0.0%
        44%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_A4a
        0.0%
        46%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_A5a
        0.0%
        46%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_A6a
        0.0%
        44%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_A7a
        0.0%
        46%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_A8a
        0.0%
        41%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_A9a
        0.0%
        44%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_B10a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_B11a
        0.0%
        39%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_B1a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_B2a
        0.0%
        48%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_B3a
        0.0%
        45%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_B4a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_B5a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_B6a
        0.0%
        27%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_B7a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_B8a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_B9a
        0.0%
        39%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_C10a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_C1a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_C2a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_C3a
        0.0%
        42%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_C4a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_C5a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_C6a
        0.0%
        51%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_C7a
        0.0%
        39%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_C8a
        0.0%
        43%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_C9a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_D10a
        0.0%
        52%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_D1a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_D2a
        0.0%
        43%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_D3a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_D4a
        0.0%
        49%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_D5a
        0.0%
        48%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_D6a
        0.0%
        49%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_D7a
        0.0%
        49%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_D8a
        0.0%
        57%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_D9a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_E10a
        0.0%
        47%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_E1a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_E2a
        0.0%
        46%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_E3a
        0.0%
        43%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_E4a
        0.0%
        45%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_E5a
        0.0%
        44%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_E6a
        0.0%
        48%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_E7a
        0.0%
        43%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_E8a
        0.0%
        49%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_E9a
        0.0%
        54%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_F10a
        0.0%
        48%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_F1a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_F2a
        0.0%
        46%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_F3a
        0.0%
        55%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_F4a
        0.0%
        50%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_F5a
        0.0%
        49%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_F6a
        0.0%
        54%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_F7a
        0.0%
        39%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_F8a
        0.0%
        48%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_F9a
        0.0%
        57%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_G10a
        0.0%
        49%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_G1a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_G2a
        0.0%
        47%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_G3a
        0.0%
        46%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_G4a
        0.0%
        45%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_G5a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_G6a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_G7a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_G8a
        0.0%
        60%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_G9a
        0.0%
        39%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_H10a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_H1a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_H2a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_H3a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_H4a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_H5a
        0.0%
        46%
        151 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_H6a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_H7a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_H8a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_H9a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A10a
        89.6%
        44%
        151 bp
        1.1
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A11a
        90.4%
        46%
        151 bp
        0.8
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A1a
        83.7%
        44%
        151 bp
        1.1
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A2a
        88.1%
        49%
        151 bp
        1.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A3a
        90.4%
        46%
        151 bp
        1.3
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A4a
        89.1%
        47%
        151 bp
        1.8
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A5a
        78.8%
        48%
        151 bp
        1.2
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A6a
        87.8%
        51%
        151 bp
        1.6
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A7a
        88.4%
        51%
        151 bp
        1.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A8a
        90.0%
        52%
        151 bp
        1.2
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A9a
        91.8%
        46%
        151 bp
        1.7
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B10a
        88.6%
        46%
        151 bp
        1.4
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B11a
        89.0%
        46%
        151 bp
        0.8
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B1a
        87.2%
        46%
        151 bp
        1.3
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B2a
        88.2%
        46%
        151 bp
        1.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B3a
        88.6%
        46%
        151 bp
        1.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B4a
        93.2%
        52%
        151 bp
        1.2
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B5a
        69.8%
        46%
        151 bp
        0.9
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B6a
        88.4%
        52%
        151 bp
        1.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B7a
        89.8%
        47%
        151 bp
        1.6
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B8a
        88.5%
        46%
        151 bp
        1.1
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B9a
        81.8%
        48%
        151 bp
        0.6
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C10a
        90.6%
        46%
        151 bp
        1.1
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C1a
        87.6%
        46%
        151 bp
        1.2
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C2a
        88.7%
        45%
        151 bp
        1.1
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C3a
        72.2%
        45%
        151 bp
        1.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C4a
        88.8%
        47%
        151 bp
        1.2
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C5a
        87.6%
        48%
        151 bp
        1.8
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C6a
        85.4%
        48%
        151 bp
        1.3
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C7a
        84.4%
        46%
        151 bp
        0.3
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C8a
        89.7%
        46%
        151 bp
        1.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C9a
        87.8%
        47%
        151 bp
        1.1
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D10a
        91.0%
        44%
        151 bp
        0.5
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D1a
        88.6%
        46%
        151 bp
        1.2
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D2a
        88.1%
        43%
        151 bp
        0.8
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D3a
        89.9%
        45%
        151 bp
        1.5
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D4a
        86.0%
        45%
        151 bp
        0.5
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D5a
        86.0%
        46%
        151 bp
        0.6
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D6a
        87.7%
        50%
        151 bp
        1.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D7a
        89.0%
        46%
        151 bp
        0.5
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D8a
        90.2%
        49%
        151 bp
        0.9
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D9a
        89.5%
        46%
        151 bp
        1.2
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E10a
        93.2%
        45%
        151 bp
        1.7
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E1a
        88.2%
        46%
        151 bp
        1.2
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E2a
        88.2%
        45%
        151 bp
        0.7
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E3a
        90.9%
        48%
        151 bp
        3.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E4a
        89.1%
        47%
        151 bp
        1.6
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E5a
        85.0%
        47%
        151 bp
        0.7
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E6a
        89.1%
        53%
        151 bp
        1.1
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E7a
        81.7%
        51%
        151 bp
        1.1
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E8a
        88.5%
        49%
        151 bp
        1.4
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E9a
        88.2%
        46%
        151 bp
        0.8
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F10a
        92.2%
        47%
        151 bp
        2.4
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F1a
        88.0%
        46%
        151 bp
        1.3
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F2a
        89.1%
        46%
        151 bp
        1.3
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F3a
        70.3%
        47%
        151 bp
        1.3
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F4a
        89.7%
        47%
        151 bp
        1.5
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F5a
        88.7%
        47%
        151 bp
        1.1
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F6a
        83.2%
        48%
        151 bp
        0.9
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F7a
        91.3%
        48%
        151 bp
        0.7
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F8a
        89.5%
        48%
        151 bp
        1.3
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F9a
        87.8%
        46%
        151 bp
        0.9
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G10a
        89.1%
        46%
        151 bp
        1.5
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G1a
        89.7%
        47%
        151 bp
        1.7
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G2a
        88.6%
        46%
        151 bp
        1.4
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G3a
        90.8%
        45%
        151 bp
        2.4
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G4a
        93.5%
        51%
        151 bp
        1.3
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G5a
        72.7%
        47%
        151 bp
        1.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G6a
        89.2%
        47%
        151 bp
        1.4
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G7a
        88.5%
        47%
        151 bp
        1.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G8a
        89.4%
        47%
        151 bp
        1.5
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G9a
        87.3%
        46%
        151 bp
        0.7
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H10a
        89.7%
        46%
        151 bp
        1.6
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H1a
        77.1%
        45%
        151 bp
        0.9
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H2a
        89.0%
        52%
        151 bp
        1.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H3a
        90.2%
        52%
        151 bp
        1.4
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H4a
        85.0%
        46%
        151 bp
        0.5
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H5a
        77.0%
        48%
        151 bp
        0.9
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H6a
        87.9%
        45%
        151 bp
        0.7
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H7a
        92.5%
        43%
        151 bp
        2.2
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H8a
        88.2%
        54%
        151 bp
        1.0
        H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H9a
        86.1%
        48%
        151 bp
        0.6
        H7GWJBGXV_n01_undetermined
        91.0%
        45%
        151 bp
        84.1
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_A10a
        0.0%
        43%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_A11a
        0.0%
        41%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_A1a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_A2a
        0.0%
        44%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_A3a
        0.0%
        42%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_A4a
        0.0%
        43%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_A5a
        0.0%
        35%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_A6a
        0.0%
        35%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_A7a
        0.0%
        39%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_A8a
        0.0%
        38%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_A9a
        0.0%
        42%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_B10a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_B11a
        0.0%
        41%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_B1a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_B2a
        0.0%
        42%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_B3a
        0.0%
        37%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_B4a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_B5a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_B6a
        0.0%
        47%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_B7a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_B8a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_B9a
        0.0%
        41%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_C10a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_C1a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_C2a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_C3a
        0.0%
        41%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_C4a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_C5a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_C6a
        0.0%
        43%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_C7a
        0.0%
        39%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_C8a
        0.0%
        43%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_C9a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_D10a
        0.0%
        50%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_D1a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_D2a
        0.0%
        41%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_D3a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_D4a
        0.0%
        50%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_D5a
        0.0%
        49%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_D6a
        0.0%
        47%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_D7a
        0.0%
        45%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_D8a
        0.0%
        49%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_D9a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_E10a
        0.0%
        43%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_E1a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_E2a
        0.0%
        43%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_E3a
        0.0%
        42%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_E4a
        0.0%
        42%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_E5a
        0.0%
        44%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_E6a
        0.0%
        47%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_E7a
        0.0%
        45%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_E8a
        0.0%
        45%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_E9a
        0.0%
        50%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_F10a
        0.0%
        42%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_F1a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_F2a
        0.0%
        50%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_F3a
        0.0%
        53%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_F4a
        0.0%
        48%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_F5a
        0.0%
        52%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_F6a
        0.0%
        55%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_F7a
        0.0%
        37%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_F8a
        0.0%
        50%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_F9a
        0.0%
        50%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_G10a
        0.0%
        43%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_G1a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_G2a
        0.0%
        46%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_G3a
        0.0%
        45%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_G4a
        0.0%
        47%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_G5a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_G6a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_G7a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_G8a
        0.0%
        54%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_G9a
        0.0%
        37%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_H10a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_H1a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_H2a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_H3a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_H4a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_H5a
        0.0%
        45%
        151 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_H6a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_H7a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_H8a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R1_H9a
        0.0%
        0%
        0 bp
        0.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A10a
        88.6%
        43%
        151 bp
        1.1
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A11a
        89.6%
        44%
        151 bp
        0.8
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A1a
        82.4%
        43%
        151 bp
        1.1
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A2a
        87.3%
        47%
        151 bp
        1.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A3a
        89.5%
        45%
        151 bp
        1.3
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A4a
        87.8%
        45%
        151 bp
        1.8
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A5a
        77.5%
        47%
        151 bp
        1.2
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A6a
        86.7%
        48%
        151 bp
        1.6
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A7a
        87.2%
        48%
        151 bp
        1.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A8a
        89.2%
        50%
        151 bp
        1.2
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A9a
        90.9%
        44%
        151 bp
        1.7
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B10a
        86.9%
        45%
        151 bp
        1.4
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B11a
        88.1%
        43%
        151 bp
        0.8
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B1a
        85.9%
        45%
        151 bp
        1.3
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B2a
        86.9%
        45%
        151 bp
        1.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B3a
        87.5%
        45%
        151 bp
        1.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B4a
        92.0%
        50%
        151 bp
        1.2
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B5a
        68.3%
        45%
        151 bp
        0.9
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B6a
        86.0%
        49%
        151 bp
        1.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B7a
        88.3%
        45%
        151 bp
        1.6
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B8a
        87.2%
        45%
        151 bp
        1.1
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B9a
        80.8%
        46%
        151 bp
        0.6
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C10a
        89.4%
        45%
        151 bp
        1.1
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C1a
        86.1%
        44%
        151 bp
        1.2
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C2a
        87.5%
        44%
        151 bp
        1.1
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C3a
        71.2%
        44%
        151 bp
        1.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C4a
        87.3%
        45%
        151 bp
        1.2
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C5a
        86.1%
        46%
        151 bp
        1.8
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C6a
        84.0%
        45%
        151 bp
        1.3
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C7a
        82.8%
        44%
        151 bp
        0.3
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C8a
        88.6%
        45%
        151 bp
        1.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C9a
        86.4%
        45%
        151 bp
        1.1
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D10a
        89.9%
        42%
        151 bp
        0.5
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D1a
        87.2%
        44%
        151 bp
        1.2
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D2a
        87.6%
        42%
        151 bp
        0.8
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D3a
        88.6%
        43%
        151 bp
        1.5
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D4a
        84.3%
        44%
        151 bp
        0.5
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D5a
        84.8%
        45%
        151 bp
        0.6
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D6a
        86.4%
        47%
        151 bp
        1.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D7a
        87.7%
        44%
        151 bp
        0.5
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D8a
        89.4%
        47%
        151 bp
        0.9
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D9a
        88.2%
        44%
        151 bp
        1.2
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E10a
        92.0%
        44%
        151 bp
        1.7
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E1a
        86.7%
        45%
        151 bp
        1.2
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E2a
        88.9%
        44%
        151 bp
        0.7
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E3a
        89.2%
        46%
        151 bp
        3.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E4a
        87.7%
        46%
        151 bp
        1.6
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E5a
        84.6%
        46%
        151 bp
        0.7
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E6a
        86.3%
        51%
        151 bp
        1.1
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E7a
        80.3%
        49%
        151 bp
        1.1
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E8a
        87.1%
        47%
        151 bp
        1.4
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E9a
        86.9%
        45%
        151 bp
        0.8
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F10a
        91.0%
        45%
        151 bp
        2.4
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F1a
        86.7%
        45%
        151 bp
        1.3
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F2a
        88.1%
        45%
        151 bp
        1.3
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F3a
        69.3%
        45%
        151 bp
        1.3
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F4a
        88.3%
        46%
        151 bp
        1.5
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F5a
        87.3%
        46%
        151 bp
        1.1
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F6a
        81.9%
        46%
        151 bp
        0.9
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F7a
        90.4%
        46%
        151 bp
        0.7
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F8a
        88.7%
        47%
        151 bp
        1.3
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F9a
        86.7%
        45%
        151 bp
        0.9
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G10a
        88.0%
        44%
        151 bp
        1.5
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G1a
        88.9%
        45%
        151 bp
        1.7
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G2a
        87.8%
        44%
        151 bp
        1.4
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G3a
        89.9%
        44%
        151 bp
        2.4
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G4a
        92.6%
        48%
        151 bp
        1.3
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G5a
        71.5%
        46%
        151 bp
        1.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G6a
        88.5%
        44%
        151 bp
        1.4
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G7a
        87.5%
        45%
        151 bp
        1.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G8a
        88.5%
        45%
        151 bp
        1.5
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G9a
        86.5%
        44%
        151 bp
        0.7
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H10a
        88.7%
        45%
        151 bp
        1.6
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H1a
        76.2%
        44%
        151 bp
        0.9
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H2a
        88.2%
        51%
        151 bp
        1.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H3a
        89.4%
        51%
        151 bp
        1.4
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H4a
        84.0%
        45%
        151 bp
        0.5
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H5a
        75.9%
        48%
        151 bp
        0.9
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H6a
        86.9%
        44%
        151 bp
        0.7
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H7a
        91.6%
        41%
        151 bp
        2.2
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H8a
        87.7%
        53%
        151 bp
        1.0
        H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H9a
        85.5%
        46%
        151 bp
        0.6
        H7GWJBGXV_n02_undetermined
        89.0%
        44%
        151 bp
        84.1

        Demultiplexing Report


        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 329/329 rows and 2/2 columns.
        LibraryTotal Read CountPortion (%)
        undetermined_library
        84058984
        19.2
        CIVIR_Ferret_Flu_R1_A1a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_B1a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_C1a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_D1a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_E1a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_F1a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_G1a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_H1a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A2a
        8.0
        0.0
        CIVIR_Ferret_Flu_R1_B2a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_C2a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_D2a
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_E2a
        8.0
        0.0
        CIVIR_Ferret_Flu_R1_F2a
        4.0
        0.0
        CIVIR_Ferret_Flu_R1_G2a
        5.0
        0.0
        CIVIR_Ferret_Flu_R1_H2a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A3a
        12.0
        0.0
        CIVIR_Ferret_Flu_R1_B3a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_C3a
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_D3a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_E3a
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_F3a
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_G3a
        4.0
        0.0
        CIVIR_Ferret_Flu_R1_H3a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A4a
        12.0
        0.0
        CIVIR_Ferret_Flu_R1_B4a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_C4a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_D4a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_E4a
        3.0
        0.0
        CIVIR_Ferret_Flu_R1_F4a
        7.0
        0.0
        CIVIR_Ferret_Flu_R1_G4a
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_H4a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A5a
        5.0
        0.0
        CIVIR_Ferret_Flu_R1_B5a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_C5a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_D5a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_E5a
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_F5a
        5.0
        0.0
        CIVIR_Ferret_Flu_R1_G5a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_H5a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_A6a
        5.0
        0.0
        CIVIR_Ferret_Flu_R1_B6a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_C6a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_D6a
        3.0
        0.0
        CIVIR_Ferret_Flu_R1_E6a
        4.0
        0.0
        CIVIR_Ferret_Flu_R1_F6a
        4.0
        0.0
        CIVIR_Ferret_Flu_R1_G6a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_H6a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A7a
        7.0
        0.0
        CIVIR_Ferret_Flu_R1_B7a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_C7a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_D7a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_E7a
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_F7a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_G7a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_H7a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A8a
        5.0
        0.0
        CIVIR_Ferret_Flu_R1_B8a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_C8a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_D8a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_E8a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_F8a
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_G8a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_H8a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A9a
        13.0
        0.0
        CIVIR_Ferret_Flu_R1_B9a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_C9a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_D9a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_E9a
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_F9a
        6.0
        0.0
        CIVIR_Ferret_Flu_R1_G9a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_H9a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A10a
        10.0
        0.0
        CIVIR_Ferret_Flu_R1_B10a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_C10a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_D10a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_E10a
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_F10a
        5.0
        0.0
        CIVIR_Ferret_Flu_R1_G10a
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_H10a
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A11a
        10.0
        0.0
        CIVIR_Ferret_Flu_R1_B11a
        2.0
        0.0
        CIVIR_Ferret_Flu_R2_A1a
        1086008
        0.2
        CIVIR_Ferret_Flu_R2_B1a
        1316061
        0.3
        CIVIR_Ferret_Flu_R2_C1a
        1202761
        0.3
        CIVIR_Ferret_Flu_R2_D1a
        1165945
        0.3
        CIVIR_Ferret_Flu_R2_E1a
        1177579
        0.3
        CIVIR_Ferret_Flu_R2_F1a
        1262962
        0.3
        CIVIR_Ferret_Flu_R2_G1a
        1740248
        0.4
        CIVIR_Ferret_Flu_R2_H1a
        867862
        0.2
        CIVIR_Ferret_Flu_R2_A2a
        986143
        0.2
        CIVIR_Ferret_Flu_R2_B2a
        1001587
        0.2
        CIVIR_Ferret_Flu_R2_C2a
        1078485
        0.2
        CIVIR_Ferret_Flu_R2_D2a
        811215
        0.2
        CIVIR_Ferret_Flu_R2_E2a
        740348
        0.2
        CIVIR_Ferret_Flu_R2_F2a
        1252735
        0.3
        CIVIR_Ferret_Flu_R2_G2a
        1353484
        0.3
        CIVIR_Ferret_Flu_R2_H2a
        1004045
        0.2
        CIVIR_Ferret_Flu_R2_A3a
        1339303
        0.3
        CIVIR_Ferret_Flu_R2_B3a
        993133
        0.2
        CIVIR_Ferret_Flu_R2_C3a
        958535
        0.2
        CIVIR_Ferret_Flu_R2_D3a
        1453578
        0.3
        CIVIR_Ferret_Flu_R2_E3a
        2999197
        0.7
        CIVIR_Ferret_Flu_R2_F3a
        1301038
        0.3
        CIVIR_Ferret_Flu_R2_G3a
        2446959
        0.6
        CIVIR_Ferret_Flu_R2_H3a
        1383538
        0.3
        CIVIR_Ferret_Flu_R2_A4a
        1765305
        0.4
        CIVIR_Ferret_Flu_R2_B4a
        1196035
        0.3
        CIVIR_Ferret_Flu_R2_C4a
        1243295
        0.3
        CIVIR_Ferret_Flu_R2_D4a
        528491
        0.1
        CIVIR_Ferret_Flu_R2_E4a
        1582888
        0.4
        CIVIR_Ferret_Flu_R2_F4a
        1452021
        0.3
        CIVIR_Ferret_Flu_R2_G4a
        1279478
        0.3
        CIVIR_Ferret_Flu_R2_H4a
        484828
        0.1
        CIVIR_Ferret_Flu_R2_A5a
        1186678
        0.3
        CIVIR_Ferret_Flu_R2_B5a
        857267
        0.2
        CIVIR_Ferret_Flu_R2_C5a
        1787521
        0.4
        CIVIR_Ferret_Flu_R2_D5a
        571460
        0.1
        CIVIR_Ferret_Flu_R2_E5a
        656221
        0.1
        CIVIR_Ferret_Flu_R2_F5a
        1107294
        0.3
        CIVIR_Ferret_Flu_R2_G5a
        951528
        0.2
        CIVIR_Ferret_Flu_R2_H5a
        885203
        0.2
        CIVIR_Ferret_Flu_R2_A6a
        1558239
        0.4
        CIVIR_Ferret_Flu_R2_B6a
        1006438
        0.2
        CIVIR_Ferret_Flu_R2_C6a
        1329022
        0.3
        CIVIR_Ferret_Flu_R2_D6a
        991098
        0.2
        CIVIR_Ferret_Flu_R2_E6a
        1098792
        0.3
        CIVIR_Ferret_Flu_R2_F6a
        856931
        0.2
        CIVIR_Ferret_Flu_R2_G6a
        1367340
        0.3
        CIVIR_Ferret_Flu_R2_H6a
        655790
        0.1
        CIVIR_Ferret_Flu_R2_A7a
        973809
        0.2
        CIVIR_Ferret_Flu_R2_B7a
        1578234
        0.4
        CIVIR_Ferret_Flu_R2_C7a
        300891
        0.1
        CIVIR_Ferret_Flu_R2_D7a
        526494
        0.1
        CIVIR_Ferret_Flu_R2_E7a
        1141968
        0.3
        CIVIR_Ferret_Flu_R2_F7a
        652840
        0.1
        CIVIR_Ferret_Flu_R2_G7a
        1027200
        0.2
        CIVIR_Ferret_Flu_R2_H7a
        2180504
        0.5
        CIVIR_Ferret_Flu_R2_A8a
        1228056
        0.3
        CIVIR_Ferret_Flu_R2_B8a
        1121105
        0.3
        CIVIR_Ferret_Flu_R2_C8a
        973720
        0.2
        CIVIR_Ferret_Flu_R2_D8a
        879024
        0.2
        CIVIR_Ferret_Flu_R2_E8a
        1362568
        0.3
        CIVIR_Ferret_Flu_R2_F8a
        1335062
        0.3
        CIVIR_Ferret_Flu_R2_G8a
        1481454
        0.3
        CIVIR_Ferret_Flu_R2_H8a
        1027447
        0.2
        CIVIR_Ferret_Flu_R2_A9a
        1652321
        0.4
        CIVIR_Ferret_Flu_R2_B9a
        631195
        0.1
        CIVIR_Ferret_Flu_R2_C9a
        1138029
        0.3
        CIVIR_Ferret_Flu_R2_D9a
        1236099
        0.3
        CIVIR_Ferret_Flu_R2_E9a
        845311
        0.2
        CIVIR_Ferret_Flu_R2_F9a
        902710
        0.2
        CIVIR_Ferret_Flu_R2_G9a
        719961
        0.2
        CIVIR_Ferret_Flu_R2_H9a
        584083
        0.1
        CIVIR_Ferret_Flu_R2_A10a
        1108164
        0.3
        CIVIR_Ferret_Flu_R2_B10a
        1411377
        0.3
        CIVIR_Ferret_Flu_R2_C10a
        1140185
        0.3
        CIVIR_Ferret_Flu_R2_D10a
        493022
        0.1
        CIVIR_Ferret_Flu_R2_E10a
        1670763
        0.4
        CIVIR_Ferret_Flu_R2_F10a
        2376747
        0.5
        CIVIR_Ferret_Flu_R2_G10a
        1508622
        0.3
        CIVIR_Ferret_Flu_R2_H10a
        1631103
        0.4
        CIVIR_Ferret_Flu_R2_A11a
        752680
        0.2
        CIVIR_Ferret_Flu_R2_B11a
        767459
        0.2
        CIVIR_Ferret_Flu_R1_A1
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_B1
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_C1
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_D1
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_E1
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_F1
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_G1
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_H1
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A2
        23.0
        0.0
        CIVIR_Ferret_Flu_R1_B2
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_C2
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_D2
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_E2
        17.0
        0.0
        CIVIR_Ferret_Flu_R1_F2
        17.0
        0.0
        CIVIR_Ferret_Flu_R1_G2
        18.0
        0.0
        CIVIR_Ferret_Flu_R1_H2
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_A3
        24.0
        0.0
        CIVIR_Ferret_Flu_R1_B3
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_C3
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_D3
        4.0
        0.0
        CIVIR_Ferret_Flu_R1_E3
        5.0
        0.0
        CIVIR_Ferret_Flu_R1_F3
        19.0
        0.0
        CIVIR_Ferret_Flu_R1_G3
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_H3
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A4
        44.0
        0.0
        CIVIR_Ferret_Flu_R1_B4
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_C4
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_D4
        5.0
        0.0
        CIVIR_Ferret_Flu_R1_E4
        13.0
        0.0
        CIVIR_Ferret_Flu_R1_F4
        27.0
        0.0
        CIVIR_Ferret_Flu_R1_G4
        3.0
        0.0
        CIVIR_Ferret_Flu_R1_H4
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A5
        16.0
        0.0
        CIVIR_Ferret_Flu_R1_B5
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_C5
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_D5
        10.0
        0.0
        CIVIR_Ferret_Flu_R1_E5
        8.0
        0.0
        CIVIR_Ferret_Flu_R1_F5
        20.0
        0.0
        CIVIR_Ferret_Flu_R1_G5
        3.0
        0.0
        CIVIR_Ferret_Flu_R1_H5
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A6
        29.0
        0.0
        CIVIR_Ferret_Flu_R1_B6
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_C6
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_D6
        3.0
        0.0
        CIVIR_Ferret_Flu_R1_E6
        11.0
        0.0
        CIVIR_Ferret_Flu_R1_F6
        25.0
        0.0
        CIVIR_Ferret_Flu_R1_G6
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_H6
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_A7
        17.0
        0.0
        CIVIR_Ferret_Flu_R1_B7
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_C7
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_D7
        5.0
        0.0
        CIVIR_Ferret_Flu_R1_E7
        13.0
        0.0
        CIVIR_Ferret_Flu_R1_F7
        20.0
        0.0
        CIVIR_Ferret_Flu_R1_G7
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_H7
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A8
        21.0
        0.0
        CIVIR_Ferret_Flu_R1_B8
        4.0
        0.0
        CIVIR_Ferret_Flu_R1_C8
        4.0
        0.0
        CIVIR_Ferret_Flu_R1_D8
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_E8
        3.0
        0.0
        CIVIR_Ferret_Flu_R1_F8
        15.0
        0.0
        CIVIR_Ferret_Flu_R1_G8
        3.0
        0.0
        CIVIR_Ferret_Flu_R1_H8
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A9
        29.0
        0.0
        CIVIR_Ferret_Flu_R1_B9
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_C9
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_D9
        3.0
        0.0
        CIVIR_Ferret_Flu_R1_E9
        4.0
        0.0
        CIVIR_Ferret_Flu_R1_F9
        24.0
        0.0
        CIVIR_Ferret_Flu_R1_G9
        2.0
        0.0
        CIVIR_Ferret_Flu_R1_H9
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A10
        8.0
        0.0
        CIVIR_Ferret_Flu_R1_B10
        1.0
        0.0
        CIVIR_Ferret_Flu_R1_C10
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_D10
        3.0
        0.0
        CIVIR_Ferret_Flu_R1_E10
        5.0
        0.0
        CIVIR_Ferret_Flu_R1_F10
        23.0
        0.0
        CIVIR_Ferret_Flu_R1_G10
        4.0
        0.0
        CIVIR_Ferret_Flu_R1_H10
        0.0
        0.0
        CIVIR_Ferret_Flu_R1_A11
        27.0
        0.0
        CIVIR_Ferret_Flu_R1_B11
        0.0
        0.0
        CIVIR_Ferret_Flu_R2_A1
        5.0
        0.0
        CIVIR_Ferret_Flu_R2_B1
        4.0
        0.0
        CIVIR_Ferret_Flu_R2_C1
        1.0
        0.0
        CIVIR_Ferret_Flu_R2_D1
        7.0
        0.0
        CIVIR_Ferret_Flu_R2_E1
        2.0
        0.0
        CIVIR_Ferret_Flu_R2_F1
        6.0
        0.0
        CIVIR_Ferret_Flu_R2_G1
        7.0
        0.0
        CIVIR_Ferret_Flu_R2_H1
        4.0
        0.0
        CIVIR_Ferret_Flu_R2_A2
        2589233
        0.6
        CIVIR_Ferret_Flu_R2_B2
        5181178
        1.2
        CIVIR_Ferret_Flu_R2_C2
        3792814
        0.9
        CIVIR_Ferret_Flu_R2_D2
        3510143
        0.8
        CIVIR_Ferret_Flu_R2_E2
        3510637
        0.8
        CIVIR_Ferret_Flu_R2_F2
        3621472
        0.8
        CIVIR_Ferret_Flu_R2_G2
        3955085
        0.9
        CIVIR_Ferret_Flu_R2_H2
        3243724
        0.7
        CIVIR_Ferret_Flu_R2_A3
        2992912
        0.7
        CIVIR_Ferret_Flu_R2_B3
        4049555
        0.9
        CIVIR_Ferret_Flu_R2_C3
        3759067
        0.9
        CIVIR_Ferret_Flu_R2_D3
        2098903
        0.5
        CIVIR_Ferret_Flu_R2_E3
        2429389
        0.6
        CIVIR_Ferret_Flu_R2_F3
        3238898
        0.7
        CIVIR_Ferret_Flu_R2_G3
        2999430
        0.7
        CIVIR_Ferret_Flu_R2_H3
        2924808
        0.7
        CIVIR_Ferret_Flu_R2_A4
        4151740
        0.9
        CIVIR_Ferret_Flu_R2_B4
        7414104
        1.7
        CIVIR_Ferret_Flu_R2_C4
        3934160
        0.9
        CIVIR_Ferret_Flu_R2_D4
        8995225
        2.1
        CIVIR_Ferret_Flu_R2_E4
        4389321
        1.0
        CIVIR_Ferret_Flu_R2_F4
        2911745
        0.7
        CIVIR_Ferret_Flu_R2_G4
        3005371
        0.7
        CIVIR_Ferret_Flu_R2_H4
        4022646
        0.9
        CIVIR_Ferret_Flu_R2_A5
        1448712
        0.3
        CIVIR_Ferret_Flu_R2_B5
        3820292
        0.9
        CIVIR_Ferret_Flu_R2_C5
        4373911
        1.0
        CIVIR_Ferret_Flu_R2_D5
        4659666
        1.1
        CIVIR_Ferret_Flu_R2_E5
        1593846
        0.4
        CIVIR_Ferret_Flu_R2_F5
        3742628
        0.9
        CIVIR_Ferret_Flu_R2_G5
        3584307
        0.8
        CIVIR_Ferret_Flu_R2_H5
        5283207
        1.2
        CIVIR_Ferret_Flu_R2_A6
        2640310
        0.6
        CIVIR_Ferret_Flu_R2_B6
        2863086
        0.7
        CIVIR_Ferret_Flu_R2_C6
        3325959
        0.8
        CIVIR_Ferret_Flu_R2_D6
        1943428
        0.4
        CIVIR_Ferret_Flu_R2_E6
        1714669
        0.4
        CIVIR_Ferret_Flu_R2_F6
        5322727
        1.2
        CIVIR_Ferret_Flu_R2_G6
        2571243
        0.6
        CIVIR_Ferret_Flu_R2_H6
        3539515
        0.8
        CIVIR_Ferret_Flu_R2_A7
        1961858
        0.4
        CIVIR_Ferret_Flu_R2_B7
        4083489
        0.9
        CIVIR_Ferret_Flu_R2_C7
        2580191
        0.6
        CIVIR_Ferret_Flu_R2_D7
        3261147
        0.7
        CIVIR_Ferret_Flu_R2_E7
        2974728
        0.7
        CIVIR_Ferret_Flu_R2_F7
        4000290
        0.9
        CIVIR_Ferret_Flu_R2_G7
        3009847
        0.7
        CIVIR_Ferret_Flu_R2_H7
        4660759
        1.1
        CIVIR_Ferret_Flu_R2_A8
        6519643
        1.5
        CIVIR_Ferret_Flu_R2_B8
        3095098
        0.7
        CIVIR_Ferret_Flu_R2_C8
        1969677
        0.5
        CIVIR_Ferret_Flu_R2_D8
        3398734
        0.8
        CIVIR_Ferret_Flu_R2_E8
        1586483
        0.4
        CIVIR_Ferret_Flu_R2_F8
        909827
        0.2
        CIVIR_Ferret_Flu_R2_G8
        4750521
        1.1
        CIVIR_Ferret_Flu_R2_H8
        2920309
        0.7
        CIVIR_Ferret_Flu_R2_A9
        3071254
        0.7
        CIVIR_Ferret_Flu_R2_B9
        4467748
        1.0
        CIVIR_Ferret_Flu_R2_C9
        4043104
        0.9
        CIVIR_Ferret_Flu_R2_D9
        4079951
        0.9
        CIVIR_Ferret_Flu_R2_E9
        2641920
        0.6
        CIVIR_Ferret_Flu_R2_F9
        2948865
        0.7
        CIVIR_Ferret_Flu_R2_G9
        3384369
        0.8
        CIVIR_Ferret_Flu_R2_H9
        3685454
        0.8
        CIVIR_Ferret_Flu_R2_A10
        1749565
        0.4
        CIVIR_Ferret_Flu_R2_B10
        2167998
        0.5
        CIVIR_Ferret_Flu_R2_C10
        2729621
        0.6
        CIVIR_Ferret_Flu_R2_D10
        2529816
        0.6
        CIVIR_Ferret_Flu_R2_E10
        3733563
        0.9
        CIVIR_Ferret_Flu_R2_F10
        3425301
        0.8
        CIVIR_Ferret_Flu_R2_G10
        1895541
        0.4
        CIVIR_Ferret_Flu_R2_H10
        4948381
        1.1
        CIVIR_Ferret_Flu_R2_A11
        4884709
        1.1
        CIVIR_Ferret_Flu_R2_B11
        4535579
        1.0

        Barcodes of Undetermined Reads


        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here. If your libraries are dual indexed, the two indicies are concatenated.

        Showing 20/20 rows and 2/2 columns.
        Barcode Sequence(s)CountFrequency (%)
        TCGACGTCAGCTAGAA
        19767422.0
        23.5
        TCGACGTCCTAGTCGA
        13320865.0
        15.8
        GGGGGGGGAGATCTCG
        12871872.0
        15.3
        TGCAGCTAACTCTAGG
        6974606.0
        8.3
        TGCAGCTATCTTACGC
        4824854.0
        5.7
        TGCAGCTATAAGGCTC
        4140410.0
        4.9
        TGCAGCTAATAGCCTT
        3341132.0
        4.0
        TGCAGCTATCGCATAA
        3257196.0
        3.9
        TCGACGTCTAAGGCTC
        2244365.0
        2.7
        TCGACGTCTCGCATAA
        2202966.0
        2.6
        TGCAGCTACTTAATAG
        1449133.0
        1.7
        GGGGGGGGAGCTAGAA
        205393.0
        0.2
        GGGGGGGGTCTTACGC
        194222.0
        0.2
        GGGGGGGGCTAGTCGA
        175793.0
        0.2
        GGGGGGGGTCGCATAA
        169749.0
        0.2
        GGGGGGGGACTCTAGG
        144820.0
        0.2
        GGGGGGGGAGCTCTCG
        142781.0
        0.2
        GGGGGGGGTAAGGCTC
        139888.0
        0.2
        GGGGGGGGATAGCCTT
        133889.0
        0.2
        GGGGGGGGCTTAATAG
        128163.0
        0.1

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Number of LanesTotal # of Single-End ReadsTotal # PF Reads% Undetermined% PhiX Aligned
        4.0
        488446040
        437496332
        19.2
        3.0

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Length Distribution

        All samples have sequences of a single length (151bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Overrepresented sequences

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

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