FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84058984
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT9770551.1623445270287824No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG6024750.7167288626757611No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC4979390.5923685682425094No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC3918580.46617027871762046No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC3681260.4379377223974061No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA3313710.39421247346981975No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC2847780.33878353799755656No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2732900.32511694407346153No Hit
ATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTAC2669040.31751989769469496No Hit
AGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA2535060.3015810897738188No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2483950.2955008354609663No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA1919060.22829921427553776No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA1783550.21217839130675195No Hit
GTATATAGCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTTTATTTT1774360.21108511137845776No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA1725660.20529156050708391No Hit
TCTTTATTTAGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTC1720010.2046194134347377No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT1671560.19885560358426413No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA1628340.19371397589102432No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC1570240.1868021626338001No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT1553780.1848440138177259No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG1490490.1773147769666119No Hit
ATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATT1342510.1597104718753203No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG1276740.1518862040968756No Hit
AATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATG1260660.1499732616325698No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC1250580.14877410366987068No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTG1185110.1409855251165063No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG1117810.13297924229015187No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCC1099690.13082361309529983No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCG1093790.13012172500205332No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1090700.1297541259837259No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA1063660.126537337163152No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA1048780.12476715159916756No Hit
ACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGC1010370.12019774114804908No Hit
GTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGG973830.11585079353326469No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA963970.11467780766895778No Hit
TAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGT951030.11313841242715948No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATT946650.11261734974098664No Hit
GTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTG922620.10975864281205208No Hit
CATGATAACCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATAT916710.10905556507796953No Hit
GGGTTAAGGGATGCCCAAATAGTTGATGAAACATGATTTATGTTTCTCTG864570.10285277775900789No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)