FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4913510
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGTTGTTGAACATGGATGAATGAAACCTGTCGAGTGAGAGTGGAAT333720.6791886044803002No Hit
GTTCACAGCTGCACAGGCTCTGTGACAACAAACATTTGGTGAATTGAACT290670.5915730302777444No Hit
CAACTACTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCTTTTTAGTT254120.517186288417038No Hit
CCCTCAGGGAGGGACCTCAGCATTGCCTTCCCTCGGCTCCCAGCAGTATC206110.41947609753516324No Hit
CCCTGAGGGTGTGGGTGTCAGGTGGAGCCCACTGTTCACAGCTGCACAGG193720.39425990788662285No Hit
ACCTAGGTCTCTGCACTCCAGAGCATGGGCTGTGCCCAGCACATGCTGAG163480.33271530942238847No Hit
GCTGTGAACAGTGGGCTCCACCTGACACCCACACCCTCAGGGAGGGACCT160080.32579561250511346No Hit
CTCCAGAGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGG158480.32253928454404285No Hit
GGCTAGGAACCTAGGTCTCTGCACTCCAGAGCATGGGCTGTGCCCAGCAC154610.3146630412882033No Hit
GCCCTGGTGTGCATTGGTTCTCATGCAGATCTTCAGGCTGGGAGTAAAAG146630.29842210558236376No Hit
ATCCTGGACGGGAGCAAGTCTGTGGTGGAAGCGGAGATGGTGGTGAGACC144740.29457556817834907No Hit
TCCTAGCGCAGCACTCCAGGATTCCCACTCAGCAGCAGGGCAGTGACCGA144000.29306951649635393No Hit
CTCCAGGATTCCCACTCAGCAGCAGGGCAGTGACCGACTTTTACTCCCAG142650.2903219897792006No Hit
GTGCCCGGGCAACCAGGATTGGGAACCCAGCTTTTTAGTTCAATTCACCA138560.2819980014287139No Hit
CCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATCCTGGACGG136320.27743914228321503No Hit
GCATAGCCCTGGTGTGCATTGGTTCTCATGCAGATCTTCAGGCTGGGAGT132050.2687488170371079No Hit
CCCCACAGATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCC128820.2621751049656966No Hit
AATTCATTCCACTCTCACTCGACAGGTTTCATTCATCCATGTTCAACAAC124580.25354583586885954No Hit
CACCAGGGCTATGCTGCCTCCGTGACCAAGTGGTCCCCAGGGCCTGGTCT119080.24235220850267933No Hit
GGATTGGGAACCCAGCTTTTTAGTTCAATTCACCAAATGTTTGTTGTCAC118610.24139566216411487No Hit
GACATATGCCTTCCAGCATATTCTCGTCTCCAGCACACAAATTCATTCCA112760.22948971305645047No Hit
CTCCCACCTCTCCCCACCACCCTCCTAGCGCAGCACTCCAGGATTCCCAC111040.2259891604982996No Hit
GGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGAAACAAGGCGGGTTAT110370.22462557316460127No Hit
CTGTGGCATCACCCCACAGATACTGCTGGGAGCCGAGGGAAGGCAATGCT108450.22071797961131656No Hit
CTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCTTTTTAGTTCAATTC107700.2191915758795647No Hit
GCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCATCCATACTTTGGA107340.2184589020883238No Hit
TTTTAGTTCAATTCACCAAATGTTTGTTGTCACAGAGCCTGTGCAGCTGT106440.2166272176102216No Hit
GTGGGGAGGCATCCATACTTTGGAGAAAGAGCAGAGCCCACTGTGGCATC106140.21601665611752086No Hit
AGTAGAAACAAGGCGGGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGA106020.21577243152044057No Hit
CCAGGGGACTTGGCTGCAAACCCAACAGATGATGGAGAAGACATATGCCT104950.2135947621964746No Hit
TGCTGAGACTCGGGGAGTGGGGAGGCATCCATACTTTGGAGAAAGAGCAG104520.21271962405693692No Hit
CATCCATACTTTGGAGAAAGAGCAGAGCCCACTGTGGCATCACCCCACAG103890.21143744492226535No Hit
TCCCAATCCTGGTTGCCCGGGCACAGAGTAGTTGTTGAACATGGATGAAT103220.21007385758856703No Hit
GACATATGCCTTCCGGCATATTCTCGTCTCCAGCACACAAATTCATTCCA102570.2087509743543821No Hit
TCTTCAGGCTGGGAGTAAAAGTCGGTCACTGCCCTGCTGCTGAGTGGGAA101700.20698034602555No Hit
GGGCTATGCTGCCTCCGTGACCAAGTGGTCCCCAGGGCCTGGTCTCACCA99330.20215691023321414No Hit
GGCTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGCTGGGTTCC96520.19643798425158387No Hit
GGGCAGACTGAGCTCCAGGGGACTTGGCTGCAAACCCAACAGATGATGGA96270.1959291830076666No Hit
GGTTTCATTCATCCATGTTCAACAACTACTCTGTGCCCGGGCAACCAGGA95270.19389397803199748No Hit
CTTTTACTCCCAGCCTGAAGATCTGCATGAGAACCAATGCACACCAGGGC92150.18754413850790982No Hit
CACAGAGCCTGTGCAGCTGTGAACAGTGGGCTCCACCTGACACCCACACC89360.181865916625793No Hit
GCACAGGCTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGCTGG88340.17979000755061048No Hit
GAGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCA85050.17309418318065903No Hit
GAACTAAAAAGCTGGGTTCCCAATCCTGGTTGCCCGGGCACAGAGTAGTT84760.17250397373771498No Hit
GATGAGGAATCCTGGACGGGAGCAAGTCTGTGGTGGAAGCGGAGATGGTG83920.17079440155815293No Hit
CTAATAACCCGCCTTGTTTCTACTAATAACCCGCCTTGTTTCTACTAATA83070.16906447732883417No Hit
CACAAATTCATTCCACTCTCACTCGACAGGTTTCATTCATCCATGTTCAA82160.16721244080097525No Hit
AATGAAACCTGTCGAGTGAGAGTGGAATGAATTTGTGTGCTGGAGACGAG82120.1671310326019485No Hit
CAGCTGCACAGGCTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAA81120.1650958276262794No Hit
GGCACAGAGTAGTTGTTGAACATGGATGAATGAAACCTGTCGAGTGAGAG80820.16448526613357864No Hit
GCCTGAAGATCTGCATGAGAACCAATGCACACCAGGGCTATGCTGCCTCC80180.16318273494915042No Hit
AGATCACCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATCCT80170.16316238289939372No Hit
GCATATTCTCGTCTCCAGCACACAAATTCATTCCACTCTCACTCGACAGG79190.16116788202323798No Hit
AGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCAT77860.15846105940559804No Hit
CCCCACCACCCTCCTAGCGCAGCACTCCAGGATTCCCACTCAGCAGCAGG77310.15734169666898No Hit
ATTCTCGTCTCCAGCACACAAATTCATTCCACTCTCACTCGACAGGTTTC77270.15726028846995327No Hit
CACAGATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTG77030.15677183927579266No Hit
GAGTAAAAGTCGGTCACTGCCCTGCTGCTGAGTGGGAATCCTGGAGTGCT76070.1548180424991503No Hit
TCTGTGGGGTGATGCCACAGTGGGCTCTGCTCTTTCTCCAAAGTATGGAT76040.15475698634988022No Hit
GAGGTGGGAGAGGGCAGAGGGCAGACTGAGCTCCAGGGGACTTGGCTGCA74870.15237579652834735No Hit
AGGTGGGAGAGGGCAGAGGGCAGACTGAGCTCCAGGGGACTTGGCTGCAA74730.15209086783175368No Hit
GACCAGGCCCTGGGGACCACTTGGTCACGGAGGCAGCATAGCCCTGGTGT73510.14960791776143734No Hit
CCCGGGCACAGAGTAGTTGTTGAACATGGATGAATGAAACCTGTCGAGTG73430.14944510136338382No Hit
GGAGATCACCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATC73250.14907876446776336No Hit
ACCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATCCTGGACG72940.14844785092530594No Hit
GTATGGATGCCTCCCCACTCCCCGAGTCTCAGCATGTGCTGGGCACAGCC72930.14842749887554924No Hit
GCATATGTCTTCTCCATCATCTGTTGGGTTTGCAGCCAAGTCCCCTGGAG72730.14802045788041543No Hit
CTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGCTGGGTTCCCA71950.1464329979993935No Hit
GTCACAGAGCCTGTGCAGCTGTGAACAGTGGGCTCCACCTGACACCCACA71880.14629053365109668No Hit
CCAGAGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAG71160.14482518606861491No Hit
GTTCAACAACTACTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCTTT71030.1445606094217779No Hit
ATCCTGGAGTGCTGCGCTAGGAGGGTGGTGGGGAGAGGTGGGAGAGGGCA70840.1441739204764008No Hit
GGTGTGCATTGGTTCTCATGCAGATCTTCAGGCTGGGAGTAAAAGTCGGT70040.14254575649586548No Hit
GCACATGCTGAGACTCGGGGAGTGGGGAGGCATCCATACTTTGGAGAAAG69990.14244399624708204No Hit
GTGCAGCTGTGAACAGTGGGCTCCACCTGACACCCACACCCTCAGGGAGG69450.14134498556022068No Hit
ACTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCTTTTTAGTTCAATT69320.14108040891338372No Hit
CTCAGGGAGGGACCTCAGCATTGCCTTCCCTCGGCTCCCAGCAGTATCTG68760.139940694127009No Hit
CATTTGGTGAATTGAACTAAAAAGCTGGGTTCCCAATCCTGGTTGCCCGG67960.1383125301464737No Hit
GTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCATCCATACTTTG66800.13595169237469754No Hit
ACTAATAACCCGCCTTGTTTCTACTAATAACCCGCCTTGTTTCTACTAAT66300.13493408988686295No Hit
CCCTCCTAGCGCAGCACTCCAGGATTCCCACTCAGCAGCAGGGCAGTGAC64060.13037523074136412No Hit
AGTCTGTGGTGGAAGCGGAGATGGTGGTGAGACCAGGCCCTGGGGACCAC63390.1290116434076658No Hit
GCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTGAGGGTGTGGG62680.12756664787494074No Hit
ATGCCACAGTGGGCTCTGCTCTTTCTCCAAAGTATGGATGCCTCCCCACT62560.12732242327786042No Hit
GTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATCCTGGACGGGAGCAAG62300.12679326998418647No Hit
GTATCTGTGGGGTGATGCCACAGTGGGCTCTGCTCTTTCTCCAAAGTATG62140.12646763718807938No Hit
AGCGAAAGCAGGCTAGGAACCTAGGTCTCTGCACTCCAGAGCATGGGCTG62040.1262641166905125No Hit
ATGTTCAACAACTACTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCT61810.12579601954610858No Hit
GTTGAACATGGATGAATGAAACCTGTCGAGTGAGAGTGGAATGAATTTGT61790.12575531544659518No Hit
CTGGAGAGATGAGGAATCCTGGACGGGAGCAAGTCTGTGGTGGAAGCGGA60500.12312990102798203No Hit
CTGCACTCCAGAGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAG60390.12290602848065843No Hit
GCCCACTGTGGCATCACCCCACAGATACTGCTGGGAGCCGAGGGAAGGCA60310.12274321208260489No Hit
CATACATGCAGCCTCAGTTTTGGTGATGTTATTTTTCAAGCTGGGTGGCA60030.12217335468941753No Hit
TCCATGTTCAACAACTACTCTGTGCCCGGGCAACCAGGATTGGGAACCCA59590.12127786450012314No Hit
CTCAGCAGCAGGGCAGTGACCGACTTTTACTCCCAGCCTGAAGATCTGCA59540.12117610425133966No Hit
GGGCAGAGGGCAGACTGAGCTCCAGGGGACTTGGCTGCAAACCCAACAGA58320.11869315418102334No Hit
CTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGCTGGGTTCCCAAT57680.1173906229965951No Hit
CACCAAATGTTTGTTGTCACAGAGCCTGTGCAGCTGTGAACAGTGGGCTC57180.11637302050876053No Hit
CCCAACAGATGATGGAGAAGACATATGCCTTCCAGCATATTCTCGTCTCC56890.1157828110658165No Hit
GACTCGGGGAGTGGGGAGGCATCCATACTTTGGAGAAAGAGCAGAGCCCA56540.11507048932433231No Hit
ATGCACACCAGGGCTATGCTGCCTCCGTGACCAAGTGGTCCCCAGGGCCT56380.11474485652822523No Hit
CTCCCTGAGGGTGTGGGTGTCAGGTGGAGCCCACTGTTCACAGCTGCACA56320.11462274422968509No Hit
GCTGAGGTCCCTCCCTGAGGGTGTGGGTGTCAGGTGGAGCCCACTGTTCA56310.1146023921799284No Hit
GCCCTGGGGACCACTTGGTCACGGAGGCAGCATAGCCCTGGTGTGCATTG56250.11448027988138826No Hit
CCCACACCCTCAGGGAGGGACCTCAGCATTGCCTTCCCTCGGCTCCCAGC55370.11268929950279942No Hit
GGGTGTGGGTGTCAGGTGGAGCCCACTGTTCACAGCTGCACAGGCTCTGT54850.11163099291545148No Hit
GGAGAAGACATATGCCTTCCAGCATATTCTCGTCTCCAGCACACAAATTC54780.11148852856715465No Hit
ATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTGAGGGT54660.11124430397007434No Hit
GAACCTAGGTCTCTGCACTCCAGAGCATGGGCTGTGCCCAGCACATGCTG54380.11067444657688699No Hit
CCCCAGGGCCTGGTCTCACCACCATCTCCGCTTCCACCACAGACTTGCTC53940.10977895638759258No Hit
GTTTCTACTAATAACCCGCCTTGTTTCTACTAATAACCCGCCTTGTTTCT53340.1085578334021911No Hit
ATATTCTCGTCTCCAGCACACAAATTCATTCCACTCTCACTCGACAGGTT53280.10843572110365095No Hit
GCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCATC53230.1083339608548675No Hit
GAGCAGAGCCCACTGTGGCATCACCCCACAGATACTGCTGGGAGCCGAGG52780.1074181186158164No Hit
CCCAACAGATGATGGAGAAGACATATGCCTTCCGGCATATTCTCGTCTCC52520.10688896532214241No Hit
ACCCCACAGATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTC52370.10658368457579204No Hit
ATTCCACTCTCACTCGACAGGTTTCATTCATCCATGTTCAACAACTACTC52280.10640051612798183No Hit
GATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTGAGGG51630.10507763289379689No Hit
GGAGCAAGTCTGTGGTGGAAGCGGAGATGGTGGTGAGACCAGGCCCTGGG51280.10436531115231269No Hit
GTGGTGGGGAGAGGTGGGAGAGGGCAGAGGGCAGACTGAGCTCCAGGGGA51050.1038972140079088No Hit
CTGTGGGGTGATGCCACAGTGGGCTCTGCTCTTTCTCCAAAGTATGGATG51030.10385650990839543No Hit
TCTGTTGGGTTTGCAGCCAAGTCCCCTGGAGCTCAGTCTGCCCTCTGCCC50980.10375474965961197No Hit
GCTTTTTAGTTCAATTCACCAAATGTTTGTTGTCACAGAGCCTGTGCAGC50730.10324594841569469No Hit
AAACAAGGCGGGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGAAACAA50590.102961019719101No Hit
GGTGAGACCAGGCCCTGGGGACCACTTGGTCACGGAGGCAGCATAGCCCT50560.10289996356983094No Hit
GAATGAAACCTGTCGAGTGAGAGTGGAATGAATTTGTGTGCTGGAGACGA50540.10285925947031754No Hit
TCATTCATCCATGTTCAACAACTACTCTGTGCCCGGGCAACCAGGATTGG50530.10283890742056087No Hit
ATGCTGAGGTCCCTCCCTGAGGGTGTGGGTGTCAGGTGGAGCCCACTGTT50130.1020248254302932No Hit
CTAAAAAGCTGGGTTCCCAATCCTGGTTGCCCGGGCACAGAGTAGTTGTT49930.1016177844351594No Hit
GTTGTTGAACATGGATGAATGAAACCTGTCGAGTGAGAGTGGAATGAATT49910.101577080335646No Hit
AAGCAGGAGATCACCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAG49410.10055947784781145No Hit
GGTTTCTACTAATAACCCGCCTTGTTTCTACTAATAACCCGCCTTGTTTC49330.10039666144975791No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)