FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027447
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAAAGCGCGCGCTATGACGGGCTACCTCCCACTAGGCCAGGACTATT74650.7265581582310329No Hit
CTTTAAGGAGCGCCTGAAATTCCCACGTCTCCGCCCACCGCTTGTAGTAG65270.6352639114231683No Hit
ACCATGGACCAATTGCAAGAGCTGGACCGCAAATTCGATATCGCGCAGGC56810.5529238977776957No Hit
ACCTACCGCACTTTGATGATGCGCATTGCCGCCTGCGATGTGGCGGGCAC49490.4816793469638823No Hit
GTAGTAGGCCACGTGGGATTCCAAGGTGCGCACCAATGCGCCCGACTTTC43770.4260073755629244No Hit
ACTACAAGCGGTGGGCGGAGACGTGGGAATTTCAGGCGCTCCTTAAAGCG42040.4091695240727745No Hit
GTCCTGGCCTAGTGGGAGGTAGCCCGTCATAGCGCGCGCTTTAAGGAGCG33960.330527998037855No Hit
CTCATAGGCTTCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCCCG31060.30230269785205466No Hit
CCTTGGAATCCCACGTGGCCTACTACAAGCGGTGGGCGGAGACGTGGGAA30730.2990908533481532No Hit
GTACAGGCGATGCGCCGCCGGGTGCTGGCCAATGTTCCCGCAGAGCTCAA30350.2953923657376001No Hit
GTGCTTGAGCTCTGCGGGAACATTGGCCAGCACCCGGCGGCGCATCGCCT30200.2939324364176449No Hit
ATTCCAAGGTGCGCACCAATGCGCCCGACTTTCCCTCGGGGCGCAGGTTC29820.29023394880709175No Hit
GCCTACTACAAGCGGTGGGCGGAGACGTGGGAATTTCAGGCGCTCCTTAA29720.2892606625937883No Hit
ATATAGTTCAGCTCGCCTGCGCCGCACTTGCCCATCGCCATGACTGCCAG29510.287216761545851No Hit
GTCATAGCGCGCGCTTTAAGGAGCGCCTGAAATTCCCACGTCTCCGCCCA28590.2782625283834592No Hit
GCTTTAGCTCGCGGTGCTTGAGCTCTGCGGGAACATTGGCCAGCACCCGG26410.25704488893344374No Hit
CGCCTAGCTTTAGCTCGCGGTGCTTGAGCTCTGCGGGAACATTGGCCAGC26110.2541250302935334No Hit
AAATAGTCCTGGCCTAGTGGGAGGTAGCCCGTCATAGCGCGCGCTTTAAG26110.2541250302935334No Hit
GGCCAGGACTATTTGGACCAGATTCGCCCGATGGTGTGGACGGCATCGCA25810.251205171653623No Hit
GTACATCCTCAACAAAAGACTCGCGCTGCGATGCCGTCCACACCATCGGG25210.24536545437380225No Hit
CGCCTGTACATCCTCAACAAAAGACTCGCGCTGCGATGCCGTCCACACCA24400.23748183604604423No Hit
GCGTCTCATCAGAGCGACCATGGACCAATTGCAAGAGCTGGACCGCAAAT22890.222785214225162No Hit
ATTTGGACCAGATTCGCCCGATGGTGTGGACGGCATCGCAGCGCGAGTCT22740.2213252849052068No Hit
CATCAGAGCGACCATGGACCAATTGCAAGAGCTGGACCGCAAATTCGATA22580.21976802696392125No Hit
GTTTAATCGCATTGGATCCACCACCTTCTTCGAGGTGGATGCGAACCTGC22570.2196706983425909No Hit
ATTCGATATCGCGCAGGCCGCCCACGCCTAGCTTTAGCTCGCGGTGCTTG22100.21509625314006464No Hit
GTCCATGGTCGCTCTGATGAGACGCTGCGCGCGCTTTCCACGGTGGAGGC21990.21402563830543084No Hit
CGTAAGGGCCAGACCAAGCTGCAGCCGGAGGTAGGATTTCGCCAGATTAC21900.21314968071345772No Hit
CCTGTACATCCTCAACAAAAGACTCGCGCTGCGATGCCGTCCACACCATC21550.2097431789668956No Hit
GGTGTGGACGGCATCGCAGCGCGAGTCTTTTGTTGAGGATGTACAGGCGA20970.20409811892973553No Hit
GAGCTGGACCGCAAATTCGATATCGCGCAGGCCGCCCACGCCTAGCTTTA20740.20185956063913763No Hit
TCTTCGAGGTGGATGCGAACCTGCGCCCCGAGGGAAAGTCGGGCGCATTG20430.19884237337789687No Hit
GCTTGTAGTAGGCCACGTGGGATTCCAAGGTGCGCACCAATGCGCCCGAC19690.19164005539945125No Hit
GTCCACACCATCGGGCGAATCTGGTCCAAATAGTCCTGGCCTAGTGGGAG19670.19144539815679057No Hit
GAAATATAGTTCAGCTCGCCTGCGCCGCACTTGCCCATCGCCATGACTGC18820.18317246534371115No Hit
GCTGGGTGCCGTCCTCGCGGCCGACGTACCCCGCTGAAATCAGCGCCTCC18810.1830751367223808No Hit
CTGCGGGAACATTGGCCAGCACCCGGCGGCGCATCGCCTGTACATCCTCA18660.18161520740242562No Hit
GGACTATTTGGACCAGATTCGCCCGATGGTGTGGACGGCATCGCAGCGCG18250.17762473392788145No Hit
CTTTGATGATGCGCATTGCCGCCTGCGATGTGGCGGGCACCTTCCATTCA17840.17363426045333724No Hit
TCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCCCGCTGAAATCAG17710.17236898837604275No Hit
GCGCAGGCAAGGGAGGCGGTTAAGGCGGCGTCGGCAAGCGCGGTGGTCAG17610.1713957021627393No Hit
GCGCACGACCTACCGCACTTTGATGATGCGCATTGCCGCCTGCGATGTGG17580.17110371629874827No Hit
ATGTACAGGCGATGCGCCGCCGGGTGCTGGCCAATGTTCCCGCAGAGCTC17380.16915714387214134No Hit
AACTCATAGGCTTCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCC17300.16837851490149855No Hit
ACCGTGGAAAGCGCGCGCAGCGTCTCATCAGAGCGACCATGGACCAATTG17180.16721057144553442No Hit
GTGCGCACCAATGCGCCCGACTTTCCCTCGGGGCGCAGGTTCGCATCCAC16950.16497201315493645No Hit
GGTCCAAATAGTCCTGGCCTAGTGGGAGGTAGCCCGTCATAGCGCGCGCT16780.16331742659232057No Hit
CCTTCCATTCACGTAAGGGCCAGACCAAGCTGCAGCCGGAGGTAGGATTT16690.16244146900034748No Hit
CGCTATGACGGGCTACCTCCCACTAGGCCAGGACTATTTGGACCAGATTC16180.15747770931249983No Hit
ATCCAATGCGATTAAACTCCGCCGCCAGCCGCGTGGCTCGAGGGGTTGCC16160.1572830520698391No Hit
CTACAAGCGGTGGGCGGAGACGTGGGAATTTCAGGCGCTCCTTAAAGCGC16030.15601777999254463No Hit
AGCTAGGCGTGGGCGGCCTGCGCGATATCGAATTTGCGGTCCAGCTCTTG15910.1548498365365805No Hit
CACTAGGCCAGGACTATTTGGACCAGATTCGCCCGATGGTGTGGACGGCA15690.15270860686731286No Hit
ACCTCCCACTAGGCCAGGACTATTTGGACCAGATTCGCCCGATGGTGTGG15550.151346006168688No Hit
GGTGCGCACCTTGGAATCCCACGTGGCCTACTACAAGCGGTGGGCGGAGA15530.15115134892602733No Hit
CTATGACGGGCTACCTCCCACTAGGCCAGGACTATTTGGACCAGATTCGC15360.14949676236341145No Hit
GGCCTAGTGGGAGGTAGCCCGTCATAGCGCGCGCTTTAAGGAGCGCCTGA15180.1477448471794652No Hit
ATATCGAATTTGCGGTCCAGCTCTTGCAATTGGTCCATGGTCGCTCTGAT15100.14696621820882244No Hit
GCTCAAGCACCGCGAGCTAAAGCTAGGCGTGGGCGGCCTGCGCGATATCG15070.14667423234483143No Hit
GTGTGGACGGCATCGCAGCGCGAGTCTTTTGTTGAGGATGTACAGGCGAT14970.14570094613152795No Hit
CGCTTTAAGGAGCGCCTGAAATTCCCACGTCTCCGCCCACCGCTTGTAGT14920.14521430302487623No Hit
TCCAAGGGGTCATCGCCGTAAATGGTGCGCACGGCGCAGGCAAGGGAGGC14890.1449223171608852No Hit
AGTGGGAGGTAGCCCGTCATAGCGCGCGCTTTAAGGAGCGCCTGAAATTC14500.1411265009290017No Hit
ATGCGAACCTGCGCCCCGAGGGAAAGTCGGGCGCATTGGTGCGCACCTTG14440.14054252920101962No Hit
CGATTAAACTCCGCCGCCAGCCGCGTGGCTCGAGGGGTTGCCTCGCTGCC14380.13995855747303754No Hit
GGTTCGCATCCACCTCGAAGAAGGTGGTGGATCCAATGCGATTAAACTCC14380.13995855747303754No Hit
ACATTGGCCAGCACCCGGCGGCGCATCGCCTGTACATCCTCAACAAAAGA14340.13956924298771614No Hit
CCTCGAAGAAGGTGGTGGATCCAATGCGATTAAACTCCGCCGCCAGCCGC14330.1394719143663858No Hit
TGCCTGCGCCGTGCGCACCATTTACGGCGATGACCCCTTGGATGCACAGC14090.13713602745445752No Hit
GGCTTCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCCCGCTGAAA13940.13567609813450232No Hit
AAACAAGGAAGTGATGGGACCGCAGAGATGAGACCCCCAGGGATGAGATG13730.13363219708656507No Hit
CTGCGATGCCGTCCACACCATCGGGCGAATCTGGTCCAAATAGTCCTGGC13700.133340211222574No Hit
ATCCACCACCTTCTTCGAGGTGGATGCGAACCTGCGCCCCGAGGGAAAGT13460.1310043243106457No Hit
AGCTAAAGCTAGGCGTGGGCGGCCTGCGCGATATCGAATTTGCGGTCCAG13420.13061500982532434No Hit
GACGTACCCCGCTGAAATCAGCGCCTCCAGCGCCTCCACCGTGGAAAGCG13270.12915508050536914No Hit
GGATCCACCACCTTCTTCGAGGTGGATGCGAACCTGCGCCCCGAGGGAAA13210.12857110877738706No Hit
GGCCAGCACCCGGCGGCGCATCGCCTGTACATCCTCAACAAAAGACTCGC13140.12788980842807463No Hit
GTGGGAGGTAGCCCGTCATAGCGCGCGCTTTAAGGAGCGCCTGAAATTCC13020.1267218649721105No Hit
GTGGTGGATCCAATGCGATTAAACTCCGCCGCCAGCCGCGTGGCTCGAGG12990.12642987910811945No Hit
CCATCGGGCGAATCTGGTCCAAATAGTCCTGGCCTAGTGGGAGGTAGCCC12930.12584590738013737No Hit
ACAAAAGACTCGCGCTGCGATGCCGTCCACACCATCGGGCGAATCTGGTC12780.12438597806018219No Hit
CCCTCGAGCCACGCGGCTGGCGGCGGAGTTTAATCGCATTGGATCCACCA12760.12419132081752148No Hit
TCTCCGCCCACCGCTTGTAGTAGGCCACGTGGGATTCCAAGGTGCGCACC12610.1227313914975663No Hit
CCGTAAATGGTGCGCACGGCGCAGGCAAGGGAGGCGGTTAAGGCGGCGTC12590.12253673425490559No Hit
GGCCAGACCAAGCTGCAGCCGGAGGTAGGATTTCGCCAGATTACGGCGCT12430.12097947631362006No Hit
TCTCATCAGAGCGACCATGGACCAATTGCAAGAGCTGGACCGCAAATTCG12400.12068749044962902No Hit
CATTGGATCCACCACCTTCTTCGAGGTGGATGCGAACCTGCGCCCCGAGG12390.12059016182829868No Hit
GCTTGAGCTCTGCGGGAACATTGGCCAGCACCCGGCGGCGCATCGCCTGT12270.11942221837233453No Hit
GTTCCCGCAGAGCTCAAGCACCGCGAGCTAAAGCTAGGCGTGGGCGGCCT12190.11864358940169176No Hit
ATTAAACTCCGCCGCCAGCCGCGTGGCTCGAGGGGTTGCCTCGCTGCCGA11980.1165996883537545No Hit
TTGATGATGCGCATTGCCGCCTGCGATGTGGCGGGCACCTTCCATTCACG11950.11630770248976348No Hit
GTGGAAAGCGCGCGCAGCGTCTCATCAGAGCGACCATGGACCAATTGCAA11940.11621037386843312No Hit
CGCCTCCACCGTGGAAAGCGCGCGCAGCGTCTCATCAGAGCGACCATGGA11920.11601571662577241No Hit
GCATCGCAGCGCGAGTCTTTTGTTGAGGATGTACAGGCGATGCGCCGCCG11650.11338784384985308No Hit
ATGGTGTGGACGGCATCGCAGCGCGAGTCTTTTGTTGAGGATGTACAGGC11470.11163592866590685No Hit
CAACAAAAGACTCGCGCTGCGATGCCGTCCACACCATCGGGCGAATCTGG11290.10988401348196063No Hit
AGCGAAAGCAGGCGCAGAAACTCATAGGCTTCAATCAGCTGGGTGCCGTC11170.10871607002599648No Hit
GGATTCCAAGGTGCGCACCAATGCGCCCGACTTTCCCTCGGGGCGCAGGT11060.10764545519136266No Hit
GACCAATTGCAAGAGCTGGACCGCAAATTCGATATCGCGCAGGCCGCCCA11050.10754812657003232No Hit
GCTCGAGGGGTTGCCTCGCTGCCGACGAAAATGACGTCCACATCGGAAAT10940.10647751173539853No Hit
GCTTCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCCCGCTGAAAT10930.10638018311406817No Hit
CTTCGAGGTGGATGCGAACCTGCGCCCCGAGGGAAAGTCGGGCGCATTGG10810.10521223965810401No Hit
GCATTGGATCCACCACCTTCTTCGAGGTGGATGCGAACCTGCGCCCCGAG10760.10472559655145229No Hit
TTGGAATCCCACGTGGCCTACTACAAGCGGTGGGCGGAGACGTGGGAATT10580.10297368136750605No Hit
CTCCTGGGCCCTGGACCCTGGGCCCTGCCTGGGGGTCCCATCTCATCCCT10530.10248703826085434No Hit
GATGAGACGCTGCGCGCGCTTTCCACGGTGGAGGCGCTGGAGGCGCTGAT10460.10180573791154192No Hit
GACCAAGCTGCAGCCGGAGGTAGGATTTCGCCAGATTACGGCGCTGACCA10430.10151375204755088No Hit
CCCCTCGAGCCACGCGGCTGGCGGCGGAGTTTAATCGCATTGGATCCACC10420.10141642342622052No Hit
AGGATGTACAGGCGATGCGCCGCCGGGTGCTGGCCAATGTTCCCGCAGAG10380.10102710894089914No Hit
CCAATGCGCCCGACTTTCCCTCGGGGCGCAGGTTCGCATCCACCTCGAAG10330.10054046583424742No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)