FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3894118
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACC366490.9411373769361895No Hit
AGCGAAAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGA278240.7145135304066287No Hit
GTATCCGAGCTTCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAGCGAT273330.7019047702201114No Hit
GTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAA267600.6871902700431779No Hit
TCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTGCA254850.6544485811678024No Hit
GCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCT204020.5239183815179714No Hit
CCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGC194700.4999848489439714No Hit
AAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGTA192110.4933337921449735No Hit
AGCAGGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAG174560.44826582039886825No Hit
GTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCA171970.4416147635998704No Hit
ACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGAGA169590.4355029816764669No Hit
GTCCAAAGAACATCGCTTTTCAGTGACGTTGTCAGGGGAGAAGGGGGAGA168390.43242141095878445No Hit
CCTCACAAACACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCT143390.3682220210070676No Hit
AGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA119640.3072326005529365No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTG115620.2969093386487004No Hit
CATTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCC114760.29470087963436137No Hit
ATCCGAGCTTCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAGCGATGT110260.28314498944305233No Hit
CTTCTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCT109300.2806797328689064No Hit
AGATTTGGTTCTTCCTTCTAAATGAGGAATCACGATCCGAGATGCAGGGC107370.27572353996463383No Hit
GGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGTACTGCC105650.2713066219359557No Hit
GAGCAGGAGCAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCC105080.26984287584505656No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA101990.2619078312470244No Hit
CTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCT101510.2606752029599514No Hit
CTTCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGG92880.2385135735486187No Hit
GAATTTCTGAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTC92250.23689574892183543No Hit
TTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCAGTG89410.2296026982233204No Hit
GGCAGTATCCGAGCTTCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAG88830.22811327237644055No Hit
CGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCCTTCTAAAT87890.22569937531425602No Hit
CTGAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAG87780.22541689799846845No Hit
GATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATG87510.22472354458698993No Hit
GCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACAT87000.2234138770319749No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT86960.22331115800805212No Hit
ATTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCG86130.22117973826165516No Hit
TCTTTTGGACAAGATTTGGTTCTTCCTTCTAAATGAGGAATCACGATCCG85740.22017822777840837No Hit
GAACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGA84440.2168398595009191No Hit
TCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGC81500.20929001124259716No Hit
CTTTTGGACAAGATTTGGTTCTTCCTTCTAAATGAGGAATCACGATCCGA81410.209058893438771No Hit
GTAGAAACAAGGGTGTTTTTCCTCATATTTCTGAAATTCTAATCTTAAAT80090.20566916564932033No Hit
ACACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCCTTCTA77310.1985301934866894No Hit
CCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCA77310.1985301934866894No Hit
GCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTGCACATCTTTTGGACA77280.19845315421874737No Hit
CACAAACACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCC74950.19246977107524732No Hit
GTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCAGT73650.18913140279775806No Hit
GCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCTTGTCC72710.1867175057355735No Hit
GCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGTACTGCCCCAGC72690.18666614622361213No Hit
GTTCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTG71550.18373865404181383No Hit
GATTTGGTTCTTCCTTCTAAATGAGGAATCACGATCCGAGATGCAGGGCT70010.17978397162078807No Hit
GTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCA69690.1789622194294061No Hit
ATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCTTGTCCAA69380.1781661469940048No Hit
AACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGAG69130.17752415309448763No Hit
GTGCAGCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAG69020.1772416757787001No Hit
CACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCCTTCTAA68720.17647128309927948No Hit
GTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTGCCTTCATTTT67630.17367218969738463No Hit
CCCTTCTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTG67180.17251660067825372No Hit
GAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACG66810.17156644970696833No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAC65030.16699545314240605No Hit
AGCAGGAGCAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCCC64870.1665845770467151No Hit
TCTGAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTA62870.1614486258505777No Hit
CTCCTCACAAACACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTT62860.161422946094597No Hit
CTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACA62060.15936856561614207No Hit
AAACAAGGGTGTTTTTCCTCATATTTCTGAAATTCTAATCTTAAATACAT60260.15474620953961848No Hit
GGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCAGTGACGTTGTCAGGG58910.15127944248222575No Hit
AGCGAAAGCAGGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAG58880.15120240321428371No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTGCCTTC58770.15091992589849615No Hit
GATGTGCAGCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCA58410.14999545468319142No Hit
AATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGGATGTGCTCT57540.14776131591287167No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAG57250.14701660298943175No Hit
CATTAGAGCACATCCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTT56330.14465406543920858No Hit
TGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCAGTGAC55540.14262536471673432No Hit
TGTCAGGGGAGAAGGGGGAGAAGCTCGGATACTGCCCGCTGCCACTGCTC55230.14182929228133304No Hit
CTCGGATACTGCCCGCTGCCACTGCTCCTGCTCCTGCTGCTGCTCCTGCT55130.14157249472152617No Hit
CTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTC54580.14016010814258836No Hit
CCAAAGAACATCGCTTTTCAGTGACGTTGTCAGGGGAGAAGGGGGAGAAG54470.13987763082680083No Hit
GAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGAGCAGTGGCAGCGGGCAGT54330.1395181142430712No Hit
GACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCTCTC54320.13949243448709053No Hit
GCGATGTTCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGA53500.1373866944966742No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC52210.13407400597516564No Hit
CTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCTTGT52200.13404832621918494No Hit
CGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCTCTCCTCAC51860.1331752145158416No Hit
AAGCAGGGGAGTCCAAGGGATGTAACTGCCCCGAGAGGCCTCAAACTGGC51730.13284137768809268No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA51700.1327643384201506No Hit
GGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGGATG51330.1318141874488652No Hit
GAGCAGCAGCAGGAGCAGGAGCAGTGGCAGCGGGCAGTATCCGAGCTTCT51030.13104379476944458No Hit
TCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGAC50910.13073563769767635No Hit
GCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGTACTGCCCCAGCCCTG50160.12880965599912483No Hit
GCGAAAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAA50150.12878397624314414No Hit
CTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTGCAC48480.12449545699436947No Hit
TCTCCAAACGGAATTTCTGAATGTACTGCCCCAGCCCTGCATCTCGGATC47460.1218761218843394No Hit
GTTCGTCTCCAAACGGAATTTCTGAATGTACTGCCCCAGCCCTGCATCTC46840.12028397701353682No Hit
GAACATCGCTTTTCAGTGACGTTGTCAGGGGAGAAGGGGGAGAAGCTCGG46600.11966766287000036No Hit
CAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCCCTGACAACG45890.1178444001953716No Hit
GTCCAAGGGATGTAACTGCCCCGAGAGGCCTCAAACTGGCCCTTTCGTGG45690.11733080507575785No Hit
AAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCT45480.11679153020016343No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTAT45070.11573866020495527No Hit
GCGAAAGCAGGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGC44980.11550754240112909No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG44920.11535346386524496No Hit
GGGCTGGGGCAGTACATTCAGAAATTCCGTTTGGAGACGAACTCCGGCAA44740.1148912282575926No Hit
GTCAGGGGAGAAGGGGGAGAAGCTCGGATACTGCCCGCTGCCACTGCTCC44600.11453171167386299No Hit
TTTCTGAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATT44120.11329908338679003No Hit
GAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAA43710.11224621339158186No Hit
AGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCCTTCTAAATGAGGAA43660.11211781461167843No Hit
TGATTCCTCATTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATGTGCAG43400.11145014095618058No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT43240.1110392648604896No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA42330.10870240706624709No Hit
ATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGG42110.10813745243467199No Hit
AGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA41610.10685346463563765No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG41350.10618579098013979No Hit
ATGTGCAGCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAA40750.10464500562129858No Hit
ATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGAGAGGCGA40460.10390029269785866No Hit
ACCAAAATGAAGGCAATACTAGTAGTTCTGGATGTGCTCTAATGGGTCTC40280.10343805709020631No Hit
CAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGAGCAGTGGCAGCG40250.10336101782226426No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT40220.10328397855432218No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT40120.10302718099451531No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT40010.10274470367872776No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT39610.10171751343950029No Hit
AAAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGT39450.10130663734380932No Hit
AAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGGATGTGCTC39420.10122959807586725No Hit
GAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGA39400.10117823856390588No Hit
GAGCAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCCCTGACA39080.10035648637252388No Hit
TCAGTGACGTTGTCAGGGGAGAAGGGGGAGAAGCTCGGATACTGCCCGCT39000.10015104832467839No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)