FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180504
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGACTCAG160880.7378110748707639No Hit
GTATTTAACATGGGAGATGTTTAATTTGGTCCCGGCAAAATTTTGAATTC129610.5944038625932353No Hit
AAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCAC103710.47562398417980434No Hit
GTCAGAGAGAGAGCAAAAGAGAGAGAGAAAGAGAGCAAGAGCGCGAGCAA84800.38890091465092474No Hit
GTTAAATACTATGGCTTAAAACATTAAGCCACCAATACCAAGATTTATTG82740.37945355752614995No Hit
GTATATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGAC76000.348543272564508No Hit
GAATATAGTCAAAGCAGTGGATACAAAGTGTCAGATACTGCTCCAGGCCA73600.3375366429045762No Hit
CAATAATGGAGGAGTGGCAGGCAGAGGTAGAAGTAGGCTCCCTGCTGAGC67750.31070798310849235No Hit
GTATTACATATTTCCATGGGAGCAGTATGATGATACAAGTATGCCTCATT65820.3018568184236305No Hit
GTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCA65090.29850896856873455No Hit
CCATAAAAGAGCATAAAAATGAATATAACTGAAGCAAACCTGAGTTAGAA62620.28718131221038806No Hit
ATATTATTGCTTCCACTATCAGGATTATTACCACCACCAAAAGGTGGCAT62500.28663098072739146No Hit
GTGAAAGATAGCCCATAGTACCTACTAGTTCCTACTCTTATTTCTCAGTC61420.28167799738042215No Hit
CACTTATTTAGTCCTCAAATATGTCTAAGAAATAGGTACTGTTCTTTTTC60150.2758536558520415No Hit
ATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTTCAGCTC59970.2750281586275467No Hit
CAATAAATCTTGGTATTGGTGGCTTAATGTTTTAAGCCATAGTATTTAAC57770.2649387481059425No Hit
ACCTAAGTGCATAGCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGA57570.2640215289676148No Hit
ATGTAATACCGATACATTTTAACTTGGCCTAAGGGATTTTTAAAATTATG57510.26374636322611655No Hit
ATAATAACTAGGATGCAGTCAGCTACACTCAATTCTCTAGTTAATATACT57480.2636087803553674No Hit
GTAGAGACAGTGAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAA54840.25150148772944236No Hit
GTACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATG53400.24489750993348328No Hit
TACCAAAAGTGCTTAAATATTATTGCTTCCACTATCAGGATTATTACCAC52430.24044899711259415No Hit
GTTAATATACTTTGTTGGCACATCAGCCTTGAAATACCCAGAATTCAAAA51030.23402846314430056No Hit
GTAGTAATGTAGATTACTTTTTAGTAAAGGTAGTTTACTTGTCAGTTGAT50490.2315519714708159No Hit
GCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGAGAATATAGTCAAA50260.23049716946173912No Hit
TTATTTATGTATGCAATAAATCTTGGTATTGGTGGCTTAATGTTTTAAGC50040.22948822840957872No Hit
GTACTATTTGATATGATGACTTAGAAAATTTTTGTTCCATTCTTCTAACT49860.2286627311850838No Hit
GTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAGTTCC47750.21898606927572709No Hit
GTATGATGATACAAGTATGCCTCATTGGATAAAAGCCACTAGCCCCATTC47670.218619181620396No Hit
CACATAATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTT46790.21458341741175435No Hit
CATAAATAATGAAACTACCTAAGTGCATAGCTAATAGTTCTATTTAAGGA46670.21403308592875775No Hit
AAATAGTACAATGCTTTTCCAGTTTTTTTCTTACTCCTTACACCCAAAAA45180.20719980334821675No Hit
TCATCATACTGCTCCCATGGAAATATGTAATACCGATACATTTTAACTTG44270.20302645626882593No Hit
GGATTATTACCACCACCAAAAGGTGGCATTGATGACCAGAAGAATTTGGA43910.20137546181983615No Hit
ATCCATAAAAGAGCATAAAAATGAATATAACTGAAGCAAACCTGAGTTAG43310.19862380440485317No Hit
GTGTATAATGATAAAATCTGTACTTTAAAACAAGCAAAATTAAGTTGTCA43040.19738555856811088No Hit
GTATATTAACTAGAGAATTGAGTGTAGCTGACTGCATCCTAGTTATTATT41640.1909650245998173No Hit
CTAAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCC41450.19009366641840603No Hit
AGTCAAAGCAGTGGATACAAAGTGTCAGATACTGCTCCAGGCCATAGCAA41420.1899560835476569No Hit
GCTTTGTACCCAGTAGAGACAGTGAGTAATAGGGTGTTTGATAGTTTGTG40080.1838107153208616No Hit
ACTACAGACCTAATAAAATCAATATTTCTATGAATGGGGCTAGTGGCTTT39910.18303107905328309No Hit
GTTTCTAATCTTATGATGCAAATTAAACCAATAAGGAACAAAAGTCACTG39800.1825266085272029No Hit
AGTTATTATTATCCAACAGTGACTTTTGTTCCTTATTGGTTTAATTTGCA39580.18151766747504247No Hit
CCTTAAGACTCTTCATCTTTAAAAATTTCATTAAATTTCGGGGCGCCTGG39380.18060044833671482No Hit
TATTCATACAGATGATGTGCCAAATCTCACAGAATATTTGGCAAAACACT38940.178582566232394No Hit
GTGTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGT38780.17784879092173186No Hit
GAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAGTT38460.1763812403004076No Hit
TTTCAAGGCTGATGTGCCAACAAAGTATATTAACTAGAGAATTGAGTGTA38340.175830908817411No Hit
GTATCTGACACTTTGTATCCACTGCTTTGACTATATTCTCTGTGTATTCT38090.17468438489450147No Hit
ATTTGATATGATGACTTAGAAAATTTTTGTTCCATTCTTCTAACTCAGGT37990.17422577532533762No Hit
ACATTACTACAGACCTAATAAAATCAATATTTCTATGAATGGGGCTAGTG37640.17262064183326423No Hit
ATACAGATGATGTGCCAAATCTCACAGAATATTTGGCAAAACACTCAGTG37400.17151997886727105No Hit
GCATTAACCACTGGCTTTCTCAAGGCACCATCAGTCACAAAGAAACAGTT37170.17046517685819426No Hit
AAAATAAACTCACTTTAGTTAAATCTGCAAAAACCAGCATTAACCACTGG36960.16950209676295022No Hit
GTTTTAAGCCATAGTATTTAACATGGGAGATGTTTAATTTGGTCCCGGCA36460.1672090489171311No Hit
GTGCATAGCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGAGAATAT36450.1671631879602147No Hit
GTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCACTATCAG35250.1616598731302488No Hit
CTTCAGTACATAATTTTAAAAATCCCTTAGGCCAAGTTAAAATGTATCGG34980.16042162729350645No Hit
AGTTAATATACTTTGTTGGCACATCAGCCTTGAAATACCCAGAATTCAAA34900.1600547396381754No Hit
CATTACTACAGACCTAATAAAATCAATATTTCTATGAATGGGGCTAGTGG34840.1597795738966771No Hit
GTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCACTATCAGGA34690.15909165954293136No Hit
GTTTAATTTGGTCCCGGCAAAATTTTGAATTCTGGGTATTTCAAGGCTGA34580.15858718901685115No Hit
GCCTTGAAATACCCAGAATTCAAAATTTTGCCGGGACCAAATTAAACATC34460.15803685753385457No Hit
CCATAGTACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTA34370.1576241089216071No Hit
CCCTATTACTCACTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAA34200.1568444726540286No Hit
ACTATTAGCTATGCACTTAGGTAGTTTCATTATTTATGTATGCAATAAAT34160.15666102882636307No Hit
ACATAATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTTC33990.15588139255878458No Hit
CACTTAGGTAGTTTCATTATTTATGTATGCAATAAATCTTGGTATTGGTG33240.1524418207900559No Hit
CTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAAAAGAAAAAAGTA33070.15166218452247737No Hit
GTACTGAAGTGATAAAATTAAAATAAACTAAGAGCCTGGAAATCTAGGAA33040.1515246016517282No Hit
GGACATGGGAGCACAATAATGGAGGAGTGGCAGGCAGAGGTAGAAGTAGG32550.14927741476282547No Hit
CAATAATATTTAAGCACTTTTGGTATGCCACATACACTTATTTAGTCCTC32470.14891052710749442No Hit
GCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCATCAATGCCAC31420.14409512663127425No Hit
ATATCAAATAGTACAATGCTTTTCCAGTTTTTTTCTTACTCCTTACACCC31120.14271929792378277No Hit
CTACTAAACTCTTTTCCTATAAGGAACTTGGAAGATTCACATTTCTTCAC30730.14093072060404385No Hit
GTTAAAATGTATCGGTATTACATATTTCCATGGGAGCAGTATGATGATAC30500.13987591859496704No Hit
GAGAATACACAGAGAATATAGTCAAAGCAGTGGATACAAAGTGTCAGATA30490.13983005763805065No Hit
GTTAAGCACCTGCCTTCAGCTCAGGGCTTGATCCGGAGTCCCATATCTGG30300.13895869945663938No Hit
GTCTTAAGGGATAAAATTAGTTTAAAGGCTTTACAAATAAATTAATAAAC30050.13781217553372982No Hit
ACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATGGA29590.13570257151557621No Hit
GATTTATTGCATACATAAATAATGAAACTACCTAAGTGCATAGCTAATAG29460.13510637907566325No Hit
TCCCAGGACCCTGAGCCAAAGGAGATGCTCAACCATCTGAGCCACCCAGG29460.13510637907566325No Hit
ATTATACACAATTCCATCTCAGAATGCATCCTGTTTATTAATTTATTTGT29430.1349687962049141No Hit
CCATTATTGTGCTCCCATGTCCCCTGCTGCTGTGCTTGCTCGCGCTCTTG28750.13185025113460008No Hit
ATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCATCAAT28680.1315292244361854No Hit
CTTATTTAGTCCTCAAATATGTCTAAGAAATAGGTACTGTTCTTTTTCCA28540.13088717103935604No Hit
GTACAAAGCACTGTGAAGAATATAAAAGAAAAAAGTAGTATTTGTAAATT28350.13001581285794478No Hit
GTGAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAG28330.129924090944112No Hit
ATCTGACACTTTGTATCCACTGCTTTGACTATATTCTCTGTGTATTCTCC28190.12928203754728265No Hit
AGTAGGTACTATGGGCTATCTTTCACTGAGTGTTTTGCCAAATATTCTGT28000.1284106793658714No Hit
GTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAGTTCCTTATAGGAAA27820.12758518214137649No Hit
GCTATGCACTTAGGTAGTTTCATTATTTATGTATGCAATAAATCTTGGTA27670.12689726778763075No Hit
CTTTTGGTATGCCACATACACTTATTTAGTCCTCAAATATGTCTAAGAAA27560.12639279726155056No Hit
GAATATAACTGAAGCAAACCTGAGTTAGAAGAATGGAACAAAAATTTTCT27520.126209353433885No Hit
ACCTATTTCTTAGACATATTTGAGGACTAAATAAGTGTATGTGGCATACC27470.1259800486493031No Hit
ATTTAAGGAAAGGAGAATACACAGAGAATATAGTCAAAGCAGTGGATACA27040.12400802750189865No Hit
GTCTGTAGTAATGTAGATTACTTTTTAGTAAAGGTAGTTTACTTGTCAGT26870.12322839123432014No Hit
GTACAATGCTTTTCCAGTTTTTTTCTTACTCCTTACACCCAAAAAATGAC26770.1227697816651563No Hit
GGTAGAAGTAGGCTCCCTGCTGAGCAAGGAGCCAGATATGGGACTCCGGA26740.12263219879440716No Hit
GATAAAATCTGTACTTTAAAACAAGCAAAATTAAGTTGTCATTTTTTGGG26480.12143981391458121No Hit
GCATTGTACTATTTGATATGATGACTTAGAAAATTTTTGTTCCATTCTTC26320.12070603860391908No Hit
GGAGAATACACAGAGAATATAGTCAAAGCAGTGGATACAAAGTGTCAGAT26310.12066017764700271No Hit
ATTCTCTAGTTAATATACTTTGTTGGCACATCAGCCTTGAAATACCCAGA26060.11951365372409314No Hit
ATCTTTCACTGAGTGTTTTGCCAAATATTCTGTGAGATTTGGCACATCAT26050.11946779276717677No Hit
ATATTAACTAGAGAATTGAGTGTAGCTGACTGCATCCTAGTTATTATTAT25410.11653269152452828No Hit
GATACAAAGTGTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATT25130.11524858473086956No Hit
TAATAATCCTGATAGTGGAAGCAATAATATTTAAGCACTTTTGGTATGCC25030.11478997516170573No Hit
ATATGGGACTCCGGATCAAGCCCTGAGCTGAAGGCAGGTGCTTAACCAAC24900.11419378272179276No Hit
TTTGAGGACTAAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTA24740.11346000741113065No Hit
GTTATATATATACAATGAACTAATTATAAAGGAATATACAATGAAAGTTA24680.11318484166963234No Hit
AATTAAACCAATAAGGAACAAAAGTCACTGTTGGATAATAATAACTAGGA24400.11190073487597363No Hit
GTACCTATTTCTTAGACATATTTGAGGACTAAATAAGTGTATGTGGCATA24260.11125868147914426No Hit
CTGTTGGATAATAATAACTAGGATGCAGTCAGCTACACTCAATTCTCTAG24180.1108917938238132No Hit
ACATATTTCCATGGGAGCAGTATGATGATACAAGTATGCCTCATTGGATA24140.11070834999614768No Hit
CATTTTAACTTGGCCTAAGGGATTTTTAAAATTATGTACTGAAGTGATAA24080.11043318425464937No Hit
CTATTACTCACTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAAAA23920.10969940894398726No Hit
CTTTAAAACAAGCAAAATTAAGTTGTCATTTTTTGGGTGTAAGGAGTAAG23860.10942424320248896No Hit
GCATCAGAATCATCTGGGATCACTTGGAGAAGTAAACTACAGATTCTGCA23710.10873632884874324No Hit
ATATTTGGCAAAACACTCAGTGAAAGATAGCCCATAGTACCTACTAGTTC23680.10859874597799407No Hit
AACTAAGAGCCTGGAAATCTAGGAATCCCTACTAAACTCTTTTCCTATAA23570.10809427545191387No Hit
TTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATGGAAAAAGAACA23560.10804841449499748No Hit
ACTATATTCTCTGTGTATTCTCCTTTCCTTAAATAGAACTATTAGCTATG23220.10648914195984047No Hit
GAAATAGGTACTGTTCTTTTTCCATTTTACAGATGAAAAGACTGAGAAAT23160.10621397621834218No Hit
GTCCCATATCTGGCTCCTTGCTCAGCAGGGAGCCTACTTCTACCTCTGCC23120.10603053239067665No Hit
TATTTGAGGACTAAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATAT22940.10520503516618177No Hit
ATTTATTTGTCAGAGAGAGAGCAAAAGAGAGAGAGAAAGAGAGCAAGAGC22880.10492986942468346No Hit
ATTCTTCACAGTGCTTTGTACCCAGTAGAGACAGTGAGTAATAGGGTGTT22850.10479228655393433No Hit
CTATCAGGATTATTACCACCACCAAAAGGTGGCATTGATGACCAGAAGAA22830.10470056464010155No Hit
ATTACTCACTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAAAAGA22530.10332473593261007No Hit
CTTTAGTTAAATCTGCAAAAACCAGCATTAACCACTGGCTTTCTCAAGGC22120.10144443669903838No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT22040.10107754904370733No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG21970.10075652234529264No Hit
GGATACAAAGTGTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAAT21880.10034377373304519No Hit
GGCCATAGCAATCTTCTCCAAATTCTTCTGGTCATCAATGCCACCTTTTG21830.10011446894846328No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)