FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6218998
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGACAGCAATTGGAGAAGACAAGACCAGCAGCAGGCTTGGGGACA194390.3125744693920146No Hit
CTGTAGAGGCACCGCCTATCCAAGCCCCCATCCTAGGCCTGGAGGGGAAC160070.25738873046751265No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT144200.2318701501431581No Hit
TCCCAGAGGAGTCCAGGGCCAAGGGAAAGTGAGACTGGAGCATCTGAGAG140400.22575984105478084No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA134590.2164175000538672No Hit
GTCCAGGGCCAAGGGAAAGTGAGACTGGAGCATCTGAGAGAAAGTAGGAG134530.2163210214893139No Hit
CTCTAGGCCTCAGCATAGTCTGGGTGTCCCCAAGCCTGCTGCTGGTCTTG125870.20239594867211724No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT122790.1974433823583799No Hit
GTCCTGCACCACTCAGCCACCTCCCTGCATCACAGGTGTGACCACAGGGA111320.17899989676793593No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT110860.1782602277730271No Hit
GGCCTAGAGAGGCTGGGCTCATTGCTGGGAACCCAACCAAAGGGGCTGGT110660.17793863255784934No Hit
GCCTTATCCCTGTGGTCACACCTGTGATGCAGGGAGGTGGCTGAGTGGTG110090.17702208619459278No Hit
GGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCT106730.17161928657960657No Hit
CCTCTACAGCGTCCCAAGACTGAATATCCAGGTAGAGCCTCCTACTTTCT101490.1631934919419495No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG98530.15843388275731876No Hit
GTCTGGGTGTCCCCAAGCCTGCTGCTGGTCTTGTCTTCTCCAATTGCTGT98020.1576138149586155No Hit
AACCAAGGGTGTCCAGGCAAGACAGGGAGGTGGCATGGGAAGGGTGGAAT93910.15100503328671275No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC90770.14595598840842205No Hit
GTCTTGGGACGCTGTAGAGGCACCGCCTATCCAAGCCCCCATCCTAGGCC89520.14394601831356113No Hit
CACCCAGACTATGCTGAGGCCTAGAGAGGCTGGGCTCATTGCTGGGAACC87690.14100342209468472No Hit
GTGTATGAGGGGGTACTCCAATGCACCGTCAACCCTGTCCTTGGTATTTA86050.13836634133022715No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT84920.13654932836447287No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA84840.13642069027840176No Hit
TCCTAGGCCTGGAGGGGAACATGACCAGCCCCTTTGGTTGGGTTCCCAGC84060.13516646893920853No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA80790.12990838717105233No Hit
TGCTTAGCCTTATCCCTGTGGTCACACCTGTGATGCAGGGAGGTGGCTGA79680.12812353372681579No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC79090.12717482784204143No Hit
GTTTGGGTGTGCCAGACTCAAACCAAGGGTGTCCAGGCAAGACAGGGAGG78190.12572764937374156No Hit
GTAGAGGCACCGCCTATCCAAGCCCCCATCCTAGGCCTGGAGGGGAACAT76690.12331568525990842No Hit
ACCCTGGACTTGCCAGACTACTGCTAAGTTTTTTGTTTGTTTGTTTCAAG73680.11847567727148328No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA72870.1171732166500134No Hit
GTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCC70140.1127834419628371No Hit
GTCCAGGGTTGGTCCCTTACTCGACCCTTCGAACATTTAAGTACAGGAAG69680.11204377296792827No Hit
GAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTG69550.11183473607806274No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTTC68840.11069307306418172No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAA68300.10982476598320179No Hit
GCTCCAGACCATCCCGGCTAACAGGGTGAAAGCCCGTCTCTAGGAAAAAT68150.10958356957181847No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC67440.10844190655793746No Hit
GATCAGGAAGGGATGACATGCTTAGCCTTATCCCTGTGGTCACACCTGTG66730.10730024354405644No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC65930.10601386268334545No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)