FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4726193
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCT76990.16290066867772857No Hit
GTCTATAACTGTGATTATTTAGCTTCTCTCTTATTCAGCCTTCTCAGGGT66820.14138229225933008No Hit
GTGTACGGAGCGCGGCTCATGGTTCCAGCTGGCCGGGGGCTCATCGTGGT62400.13203015619548333No Hit
GTTATAGACAACACAGAAGATTCTAGGTGGCACGTTCTTAAAAATCGAGG58490.12375711275438815No Hit
CCGTACACCGAGCACAAGTAGTGGCCTCTGAGTCCAACATTGTTGATATC56890.12037172413399114No Hit
GTCTAGTCTCCAGGTAAATATCATTGCTCCATGCATGCACTTTAGCCATA55640.11772688927430598No Hit
ATCTTACTTTCTGGTACAAATGGAAGATTTGATCCATAAAGAAATTCAAA54370.11503973705686585No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG52470.1110195880701444No Hit
GTCTTAAAGCGTTACCATTAAAAAGGAATTATCCTACTTAGGAAAAAAGG52320.11070220788698219No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTTC48210.10200599086833738No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)