FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885203
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGAGGAGCCAGCCTGTGGGGCAGGGAGAGGAGGGCTGCAGCACC46790.5285793202237227No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG35130.3968581218093477No Hit
CTACTGGGCTGTGGCTCCTTCTCCCCCTGGAGAATGGAGGGCCGGGTGAT27140.30659634004855385No Hit
CCCTAGGCCCAGGGGTTCCTGGTCTGTAAATGGGGAGAGACGGCCTGCAC27090.30603149785981293No Hit
TCCCTAGGCTGTGTAATTTAAGCCACAGCCCCACCTTCCCCATATCATGT26220.2962032437757215No Hit
GTGCAGTGCTGGGCAGGGGGTGAACACTCCCTGTTCCTGGGTCTTCTCCC22020.24875649992148696No Hit
GTACCAGGGATGTGAGCAGAAATGACAACGGCTTTCTAAGTGCAAGGCAA19840.22412938049238426No Hit
GGCCTAGGGAGCGTGTGTGTCTAGAGTGTGAATGGATCACAGATGAATGA18230.20594146201492763No Hit
GCACTGCACTGAGCGTTTTGTGGATTGTCTCATTCAGTGTTCACCAGTCC17840.2015356929427487No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG17840.2015356929427487No Hit
ATACAGGTCAGTGTGAATGACATCCGAATTATATTAGTGGAAAAACTTGT16920.1911425966699164No Hit
CTCTAGACACACACGCTCCCTAGGCCCAGGGGTTCCTGGTCTGTAAATGG14860.16787109849379184No Hit
GGTCAGGAGTGGGGACTTTATTCAACATGTGATGGGAAGCCTGGGGAAGG13910.15713909690771494No Hit
GTCTCACAGTGAGCAGACTGGCCTGGTGGAACTTGGAACCAACTAGAGAT13840.15634831784347772No Hit
CTGTGAGACCTGCCTGGCCCTGCTTTTGGTCTTATTAACCCACTCAGTTC13600.15363707533752144No Hit
GTGTGTATGTGTGCATGTGGGTGTGTAAACTGCTTTCCCAATACCAGTAG13440.1518295803335506No Hit
GTGCTGGGCAGGGGGTGAACACTCCCTGTTCCTGGGTCTTCTCCCTCCAA12480.1409846103097256No Hit
CCCTGGTACCTAGCAAAATGCCATGCGCACTAAATGGCCAGGATGACTCA12330.1392900837435029No Hit
GTCTAAGGGTTGACTTTTCTACAATAAGCTGCCCGATTTAATCAAGAGGA12130.13703071498853936No Hit
GTCCTTTAGTTCTCCCAACAATGCTGCAGTGCAGGCCGTCTCTCCCCATT11140.12584683965146978No Hit
TGTCTAGAGTGTGAATGGATCACAGATGAATGAACACAGAGCCCATGGGT10920.12336153402100988No Hit
TCCCAGGACCCTGGGATCATGACCTGAGCCAAAGATAGATGCTTAATGAC10810.12211888120577993No Hit
CTTTAAGCCCAGGCGTGCCGTGGTCTGATCTATGGATTTAAAAATCCTCC10650.12031138620180908No Hit
CCCATGGGCTCTGTGTTCATTCATCTGTGATCCATTCACACTCTAGACAC10370.11714826994486009No Hit
GTGTTTGAGTCATCCTGGCCATTTAGTGCGCATGGCATTTTGCTAGGTAC10020.11319437462367389No Hit
AAACTATACAGGTCAGTGTGAATGACATCCGAATTATATTAGTGGAAAAA9970.112629532434933No Hit
GCCCATGGGTCCTCATTTCCACTTGGTCCCAACTGGCTGAGGGGTGGCTG9900.11183875337069575No Hit
GTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACAAGGAATAACC9870.11149984805745124No Hit
GGGTAAAGGCTTGGGGCCCAAGGGTAGAGGAAAGATGGCTCTGAAACCAC9850.11127391118195488No Hit
GCCCAGCACTGCACTGAGCGTTTTGTGGATTGTCTCATTCAGTGTTCACC9790.1105961005554658No Hit
ATATGGGGAAGGTGGGGCTGTGGCTTAAATTACACAGCCTAGGGAGGAGC9690.10946641617798404No Hit
GTGTGAATGACATCCGAATTATATTAGTGGAAAAACTTGTTCCTACACTC9560.10799782648725773No Hit
CCTCTAAGGCAGCTTCCTACTATGGGGGTTGTTTCCCATTTAGGGACCAG9550.10788485804950955No Hit
GTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAA9320.10528658398130146No Hit
GCTCTGGACACTACTGGGCTGTGGCTCCTTCTCCCCCTGGAGAATGGAGG9320.10528658398130146No Hit
AGCAGGGACAGTGGCAGAGGGAGAAGCAGGCTCCCCACTGAGCAGGGAGC9020.10189753084885614No Hit
GCCCTGAGGACGTCAGGAGTGAAGCACATGATATGGGGAAGGTGGGGCTG8860.1000900358448853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)