FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5361949
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAA429220.8004925074818876No Hit
GGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCA403140.7518534771591449No Hit
CAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTT394550.7358331830459409No Hit
ATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTT319830.5964808691764879No Hit
TAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAG286880.5350293335501699No Hit
ATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCT278240.5189157897622674No Hit
TTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTT227650.4245657689023152No Hit
AGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAA225840.4211901306782291No Hit
GTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAG215330.40158904905660237No Hit
ATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTTGGCTCA211290.3940544753409628No Hit
CCCCAAGATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAACATGCACA210790.39312197859397763No Hit
GCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGAT208340.3885527445337507No Hit
CTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAACCTCTTAAGTG203670.37984322491690986No Hit
ATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAA197690.3686905638229681No Hit
GTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTTTC195550.36469947774587186No Hit
GCTTTATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGT171480.3198090843460093No Hit
CTTTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTC170950.31882063779420505No Hit
GATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTA165400.3084699239026705No Hit
ATTTTAGGCATCCTAGTTTGAGAAAAACATGCACAACTGAATTTGAAGAC160050.29849220870992993No Hit
CAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAA157680.29407217412922054No Hit
TATTTGTCCTCTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAAC153300.2859035026256311No Hit
GTTTAGCACCAGGTTCCCCACGAACGTGCGTTGCGTGACGGGCGAGGGGG149370.2785740781943282No Hit
TATCTAGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAGGCACCA142760.26624647119918526No Hit
GTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTT141800.26445607744497385No Hit
CTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGATGTAGCAGAG139340.25986819344980716No Hit
GTTTTATTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGC136390.2543664626425951No Hit
AGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAGGCACCATAAAA135110.2519792709703132No Hit
AGCAGGGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAGCG133850.24962937916791078No Hit
ATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAAT125750.2345229318667522No Hit
GTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTC125600.23424318284265663No Hit
GACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTTTCCCTTATTT123320.2299909976764046No Hit
GCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATCTAGATATTAA123010.22941284969327383No Hit
CTCTTTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGT121110.2258693620547305No Hit
AGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGA120850.22538446374629822No Hit
AGTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATC117090.2183720882089703No Hit
GCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTCAGCTCAGTTCCTGAC114290.21315010642585375No Hit
GCCTAAAATGGATTAATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTG113310.21132241280176295No Hit
GGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTGGTTTT112660.21011016703068233No Hit
GTACTAAGGCACCATAAAAATAAAACAGATAAAATTGGTTTTAAAAATAG109840.20485088537768636No Hit
GTGGAGAGCCTCCTTCTCCCTCTTCTGTTTCCCCTGCTTGTGCGCTCTTT103780.19354902480422698No Hit
TCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAATTCAGTTG103280.19261652805724186No Hit
TCCTGGGATTGAGCCCCACATTGAGCTCCCTGCTCAGTGGAGAGCCTCCT103010.1921129798138699No Hit
GCTCAGTGGAGAGCCTCCTTCTCCCTCTTCTGTTTCCCCTGCTTGTGCGC102180.19056503521387466No Hit
CTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAA98020.18280666227895864No Hit
GAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTG96300.1795988734693299No Hit
AGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTT95530.17816282847897283No Hit
GATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAA94420.17609268570066594No Hit
GGATAAGTAGGCTTCCCACTGAGCGGGGAGCTCCATGCAGTGCTCAATCC94090.1754772378476558No Hit
TAACAGGGATGCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTCAGCTC93640.17463799077536918No Hit
GCACTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGATGTAGCA92700.1728848968910372No Hit
ATCCCAGGACGCAGGGGTCAGGAACTGAGCTGAAGGCAGACACTTAGCCA92360.17225079910308733No Hit
ATCCCAGGAAGGACCCTGGGATCATGACCTGAGCCAAAGGCAGACACTAA91490.17062825476333324No Hit
CTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCT90890.16950925866695113No Hit
GGTAAGACTTCTGTGGTTTTAGTCAGCCATACTGTGAGGAAAGAAGAAAG90200.1682224131561117No Hit
GAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGCTC88460.16497732447660354No Hit
CCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATCTAGATATTAAT87280.16277663215371874No Hit
GAGAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGC86620.1615457364476984No Hit
CACTTAGCCAGTGGACACACCCAGGCATCCCTGTTATTAGCTATTTTTAA85790.15999779184770316No Hit
CAGCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAG83150.15507420902362182No Hit
TCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTAC82840.15449606104049107No Hit
GCTAATAACAGGGATGCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTC81760.15248186806700326No Hit
CTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTC81260.15154937132001817No Hit
CCACTGAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGG81230.15149342151519904No Hit
GCTCCATGCAGTGCTCAATCCCAGGACGCAGGGGTCAGGAACTGAGCTGA79480.1482296829007512No Hit
CACCCAGGCATCCCTGTTATTAGCTATTTTTAAAACCAATTTTATCTGTT78550.1464952389513589No Hit
GGTCATGATCCCAGGGTCCTTCCTGGGATTGAGCCCCACATTGAGCTCCC77610.14474214506702693No Hit
ATCATGACCTGAGCCAAAGGCAGACACTAACCGACCAAGCCACCTAGGCA77350.14425724675859466No Hit
CCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGGTTCCGGCGGCGT77080.14375369851522274No Hit
ATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCA76010.1417581554766746No Hit
CTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGT75460.14073240905499101No Hit
CAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATT75440.1406951091851116No Hit
GTCCTCAGTTTGTCGTCTTCAAATTCAGTTGTGCATGTTTTTCTCAAACT73620.13730082102608585No Hit
GAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGGGATCA73130.13638697421404045No Hit
ACATTGAGCTCCCTGCTCAGTGGAGAGCCTCCTTCTCCCTCTTCTGTTTC72750.13567827668633178No Hit
GGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAA72050.13437278124055263No Hit
GTTAGTGTCTGCCTTTGGCTCAGGTCATGATCCCAGGGTCCTTCCTGGGA71690.13370138358272338No Hit
GTTTTAGTCAGCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATT71180.13275023690079857No Hit
CCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTT71100.13260103742128096No Hit
GGTCAGGAACTGAGCTGAAGGCAGACACTTAGCCAGTGGACACACCCAGG69360.1293559487417728No Hit
AATCAAAAGGACTCAATGACTGGCAAGATGTAGCAGAGGACAAATAAGGG68650.128031803361054No Hit
GATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAACATGCACAACTGAA67840.1265211586309381No Hit
GGCTAAGTGTCTGCCTTCAGCTCAGTTCCTGACCCCTGCGTCCTGGGATT66660.1243204663080533No Hit
GCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTTGGCTCAGGTCATGATC66610.12422721663335479No Hit
ATTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTAT66170.1234066194960079No Hit
GATTAATATCTAGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAG65150.1215043261321583No Hit
TTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATC64970.12116862730324365No Hit
AGCGAAAGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT64960.12114997736830395No Hit
GAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGCTCTC64760.12077697866950991No Hit
GGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGG63900.11917308426469554No Hit
TTATTTGACAGAGAGAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAG63660.1187254858261427No Hit
GTCATTGAGTCCTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATT63280.11801678829843401No Hit
TCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGA62520.11659939324301666No Hit
GGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGA62190.1159839453900065No Hit
GTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATCT61850.11534984760205665No Hit
GCCTTGGAAAGCGTCGCGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGC60750.11329835475868942No Hit
GATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACT60730.11326105488881001No Hit
AACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCA60020.11193690950809117No Hit
CTCAATGACTGGCAAGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGA59390.11076196360688996No Hit
ACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGG59010.11005326607918127No Hit
CTTCTGTTTCCCCTGCTTGTGCGCTCTTTCTCTCTCTCTGTCAAATAAAA58730.10953106790086962No Hit
AGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGAC58380.10887832017798006No Hit
ACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAG56870.10606218000208506No Hit
ATCCCAGGGTCCTTCCTGGGATTGAGCCCCACATTGAGCTCCCTGCTCAG56740.10581973084786893No Hit
CAGTTTGTCGTCTTCAAATTCAGTTGTGCATGTTTTTCTCAAACTAGGAT56340.10507373345028087No Hit
GCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAACCTCTTAAGTGCT56280.10496183384064266No Hit
GGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAGCGGGGAG55610.10371228819968262No Hit
GGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGT55130.10281709132257692No Hit
GCTCTCCACTGAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGAC55110.10277979145269751No Hit
AGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTA55110.10277979145269751No Hit
TCCATGCAGTGCTCAATCCCAGGACGCAGGGGTCAGGAACTGAGCTGAAG55060.10268654177799902No Hit
TTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAATTCAGTTGTGC54960.10250004242860199No Hit
ATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGC54940.10246274255872258No Hit
ATCCTAGTTTGAGAAAAACATGCACAACTGAATTTGAAGACGACAAACTG54720.10205244399004913No Hit
CTTAAGTGCTTTTTGCATTCTATTCACTTTCTCTAATCCTACTACCACTA53960.10063504893463178No Hit
AACTAGGATGCCTAAAATGGATTAATCTTGGGGTGCCTAGGTGGCTTGGT53800.10033664997559656No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)