FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1383538
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCAGCCCCAGGGGAGC97080.7016793178069558No Hit
GTGATAATGCTGGTGGGGGCACACAGGCAGAGTCCCCACGTGCTGTAGAG72720.5256089821891412No Hit
CCATAGAGCGACCCACGAAATGTTGGCTGAATTAAAGAGTCAAGAATCTG70310.5081898726308927No Hit
GTTCTGTACTGTTCTGAATTTGTAACATCTGGTCTCTGCTCAGGCGAACA62520.45188495003389856No Hit
GGATAAGGAGGGGGCGGCTCATTAGACATCTTTGCTGCTTCTGGACCTCA56180.40606040455701253No Hit
CTTTCAAACCATCAGCTCTCCTGAGAACTCCCTCACTATCAGGGGAGCAG55510.40121774754289363No Hit
GTGCCACACAGTGAGTGACCTCCATAGAGCGACCCACGAAATGTTGGCTG55280.39955534289625577No Hit
CTCCTGAGAACTCCCTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCC52790.38155800563482895No Hit
GTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCAGCCCCAGGGG51980.37570345014014794No Hit
AATTAACACTGGCCATTAAGAAGCTGCGGTGAGTAAAACGTCAAGCTCAG51540.3725231977726669No Hit
CTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCATCCCCTCC50490.36493395916845073No Hit
GGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAA47630.3442623187798239No Hit
CCCTTGACACAGGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACAC46820.3384077632851429No Hit
GTCATAGATCATCTGGTTCTTGTGGGGTGTGTGTGTCAGAATCACATGGG44750.32344612146540247No Hit
GAACTGTACTCAGCCCCAGCCCCAGGGGAGCTGCAAGATAGACTGAGACC44310.32026586909792143No Hit
CCTGAGAACTCCCTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCC43460.31412219975165123No Hit
GTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCAGC42490.3071111888506134No Hit
GTCCCTACACATAGTGATCTCTTCTGTGCAGTATTGTTAGTGAAGCTCGT41220.2979318240626568No Hit
ATCTTGCAGCTCCCCTGGGGCTGGGGCTGAGTACAGTTCTGCTGGCCCTG40100.2898366362181595No Hit
GTGTAGGGACATGGCATCAAAGTTCTGGCAAGGAGGGGCGCCTGGGTGGC39060.28231967607684066No Hit
GCTCTAGGGACCTGTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTTTA38960.2815968914478677No Hit
GTGTTAATTTGTGTTATTTCTTAAGCAATTTACTCGATAACGTTTTGTTA36740.2655510726846679No Hit
ACTGTACTCAGCCCCAGCCCCAGGGGAGCTGCAAGATAGACTGAGACCCT33600.24285563533491672No Hit
GGGTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAAT32780.23692880137733838No Hit
CTCCAGGATAAGGAGGGGGCGGCTCATTAGACATCTTTGCTGCTTCTGGA32610.23570006750808434No Hit
GTACTCAGCCCCAGCCCCAGGGGAGCTGCAAGATAGACTGAGACCCTCAC32290.23338715669537083No Hit
CCCCTGGGGCTGGGGCTGAGTACAGTTCTGCTGGCCCTGCTCTACAGCAC32220.23288120745508975No Hit
CATTAGACATCTTTGCTGCTTCTGGACCTCAAATCCCAGGTACTCAGAAG32000.23129108127134926No Hit
TTACAATTCCAGATGAGATTTCGGTGGGGACACAGAGCCCAACCTGTGAG31010.2241355134445169No Hit
GTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCA30980.22391867805582497No Hit
CCCCAGGGGAGCTGCAAGATAGACTGAGACCCTCACAGGTTGGGCTCTGT30450.22008791952226828No Hit
TTCCAGGAGAGAGCAGGAGGCCTGACAGTTTGCGGGAGCCCTGGAGGAAA29980.21669083176609535No Hit
GCCCCAGCCCCAGGGGAGCTGCAAGATAGACTGAGACCCTCACAGGTTGG29660.21437792095338185No Hit
CTGCAAGATAGACTGAGACCCTCACAGGTTGGGCTCTGTGTCCCCACCGA29510.21329374400992238No Hit
CACAGGCAGAGTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACT28750.20780058082972785No Hit
CTATAAAACACGTCAAATGTCAAGTTTATAACAAAACGTTATCGAGTAAA27880.20151235455766306No Hit
GGCAGAGTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCAGC27810.20100640531738198No Hit
GCTAAATGCTGTATGTCATAGATCATCTGGTTCTTGTGGGGTGTGTGTGT27780.20078956992869004No Hit
TCCCTACACATAGTGATCTCTTCTGTGCAGTATTGTTAGTGAAGCTCGTT27330.19753703909831172No Hit
CCCTAGAGCCCTGCGTGTCTGCTCCGGCTGGCAGTAGAAGGAGCCTCTCC27130.1960914698403658No Hit
CTCCTCCAGTGTTCTCTGGGTGGGAGCTGGATCTACAACTTCATGAGGCC26520.1916824836036307No Hit
ACCTGTGAGGGTCTCAGTCTATCTTGCAGCTCCCCTGGGGCTGGGGCTGA26450.1911765343633496No Hit
GTGCCACACTTTCAAACCATCAGCTCTCCTGAGAACTCCCTCACTATCAG26230.1895864081796091No Hit
GGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGG26190.18929729432801992No Hit
CTTGTGGGGTGTGTGTGTCAGAATCACATGGGAAACACCTTCAGAGGGTA25780.18633387734923074No Hit
GTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCAGCCCCAGGGGAGCTG25760.18618932042343614No Hit
ACACAGGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACACAGAGCC25730.18597248503474426No Hit
GGTTGGGCTCTGTGTCCCCACCGAAATCTCATCTGGAATTGTAATCCTCC25620.185177421942874No Hit
ATGCTGGTGGGGGCACACAGGCAGAGTCCCCACGTGCTGTAGAGCAGGGC25450.18394868807361994No Hit
GAGCCCAACCTGTGAGGGTCTCAGTCTATCTTGCAGCTCCCCTGGGGCTG25250.18250311881567402No Hit
GATCCCATCCCCTCCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAA24890.17990109415137134No Hit
GGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACACAGAGCCCAACC24580.17766046180155515No Hit
CCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAATGCTG23810.1720950201584633No Hit
GTTTATAACAAAACGTTATCGAGTAAATTGCTTAAGAAATAACACAAATT23810.1720950201584633No Hit
TTAGACATCTTTGCTGCTTCTGGACCTCAAATCCCAGGTACTCAGAAGGG23710.17137223552949032No Hit
CTTCTGGACCTCAAATCCCAGGTACTCAGAAGGGGAAGGCAGGAGAGGCT23560.17028805858603088No Hit
GTATGTCATAGATCATCTGGTTCTTGTGGGGTGTGTGTGTCAGAATCACA23320.16855337547649576No Hit
GTACTCAGAAGGGGAAGGCAGGAGAGGCTCCTTCTACTGCCAGCCGGAGC23240.1679751477733174No Hit
GTTCTGAATTTGTAACATCTGGTCTCTGCTCAGGCGAACAATTTCACAAA23230.1679028693104201No Hit
GCTCTACAGCACGTGGGGACTCTGCCTGTGTGCCCCCACCAGCATTATCA23190.16761375545883092No Hit
GCTCTATGGAGGTCACTCACTGTGTGGCACCGGGCCTCATGAAGTTGTAG22960.1659513508121931No Hit
GTATTGTTAGTGAAGCTCGTTGGCCTGGCAGGATCAGGTAGTCTTGTGTC22660.16378299692527418No Hit
GTGTGGCACCGGGCCTCATGAAGTTGTAGATCCAGCTCCCACCCAGAGAA22590.1632770476849931No Hit
GAATTAAAGAGTCAAGAATCTGAAGGCTTTTCAGACAGAACAGGTCCCTA22590.1632770476849931No Hit
AGCGAAAGCAGGGTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCA22540.16291565537050662No Hit
GCTCATTAGACATCTTTGCTGCTTCTGGACCTCAAATCCCAGGTACTCAG22460.16233742766732825No Hit
GTGAGTGACCTCCATAGAGCGACCCACGAAATGTTGGCTGAATTAAAGAG22120.15987995992882018No Hit
GACCTGTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTTTAATTCAGCC22100.15973540300302558No Hit
GCCTTAGCTAAATGCTGTATGTCATAGATCATCTGGTTCTTGTGGGGTGT21890.15821755528218234No Hit
CTTCTGTGCAGTATTGTTAGTGAAGCTCGTTGGCCTGGCAGGATCAGGTA21860.15800071989349046No Hit
GCCCCAGGGGAGCTGCAAGATAGACTGAGACCCTCACAGGTTGGGCTCTG21580.15597692293236615No Hit
AAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAATGCTGGTGGG21250.15359173365675538No Hit
GGATTACAATTCCAGATGAGATTTCGGTGGGGACACAGAGCCCAACCTGT21120.1526521136390905No Hit
CCTCCATAGAGCGACCCACGAAATGTTGGCTGAATTAAAGAGTCAAGAAT20480.14802629201366352No Hit
TCCCTTGACACAGGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACA20410.14752034277338244No Hit
AGCAGGAAGTGCCACACTTTCAAACCATCAGCTCTCCTGAGAACTCCCTC20380.14730350738469056No Hit
AAAGCAGGGTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGC20270.14650844429282028No Hit
CCTCCAGTGTTCTCTGGGTGGGAGCTGGATCTACAACTTCATGAGGCCCG20260.146436165829923No Hit
TCCCAGGACCCTGGGATCATGACCCAAGCTGAAGGCAGAGGCTTTAAGCC20230.1462193304412311No Hit
ATCCCATCCCCTCCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAAT20170.14578565966384732No Hit
CAGGCAGAGTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCA19850.14347274885113384No Hit
TCCCATCCCCTCCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAATT19740.14267768575926357No Hit
GGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAATGCTGGTGGGGGCAC19660.14209945805608518No Hit
GATAAGGAGGGGGCGGCTCATTAGACATCTTTGCTGCTTCTGGACCTCAA19610.1417380657415987No Hit
TCTTTGCTGCTTCTGGACCTCAAATCCCAGGTACTCAGAAGGGGAAGGCA19580.14152123035290684No Hit
GGCCATGAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGGAGCTGGATC19540.14123211650131764No Hit
CCCCAGATCCCATCCCCTCCCACCAGGTTCCTCCCTTGACACAGGAGGAT19340.1397865472433717No Hit
CTAGGGACCTGTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTTTAATT18910.13667857333878794No Hit
ATCTACAACTTCATGAGGCCCGGTGCCACACAGTGAGTGACCTCCATAGA18860.13631718102430146No Hit
CCCCTACCCTCTGAAGGTGTTTCCCATGTGATTCTGACACACACACCCCA18440.13328148558261502No Hit
GGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCG17990.13002895475223664No Hit
GCCCAGGCCCCTACCCTCTGAAGGTGTTTCCCATGTGATTCTGACACACA17880.1292338916603664No Hit
GACTGAGACCCTCACAGGTTGGGCTCTGTGTCCCCACCGAAATCTCATCT17600.12721009469924208No Hit
ATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCATCCCCTCCCA17520.1266318669960637No Hit
CTCCCTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCA17420.12590908236709075No Hit
GCCCAGCTCCTCCCACTCTGCACCTGCTGCCTCAGACTCCCCACGCTGAG17330.12525857620101508No Hit
GTCAGGGCCAAAATCAGGAGGCGGTGATAATGCTGGTGGGGGCACACAGG17250.1246803484978367No Hit
GTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTTTAATTCAGCCAACAT17200.12431895618335023No Hit
GGGCTCTAGGGACCTGTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTT17140.12388528540596645No Hit
GTCTTGTGTCTACCTGTGTTCCTTCTTCTTCTTCTTTTTTTAACCTACAT17120.12374072848017185No Hit
GTACAGTTCTGCTGGCCCTGCTCTACAGCACGTGGGGACTCTGCCTGTGT17070.12337933616568537No Hit
GCCTCAGACTCCCCACGCTGAGCCCAGGCCCCTACCCTCTGAAGGTGTTT17010.1229456653883016No Hit
GAACAGGTCCCTAGAGCCCTGCGTGTCTGCTCCGGCTGGCAGTAGAAGGA17010.1229456653883016No Hit
CTCACAGGTTGGGCTCTGTGTCCCCACCGAAATCTCATCTGGAATTGTAA16830.12164465305615024No Hit
CTGCAGGGCCATGAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGGAGC16740.12099414689007457No Hit
CTGTGTCCCCACCGAAATCTCATCTGGAATTGTAATCCTCCTGTGTCAAG16440.11882579300315568No Hit
GCCACACAGTGAGTGACCTCCATAGAGCGACCCACGAAATGTTGGCTGAA16370.1183198437628746No Hit
CAGCAGAACTGTACTCAGCCCCAGCCCCAGGGGAGCTGCAAGATAGACTG16100.1163683252646476No Hit
CCTCCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAATTCCAGATGA15960.11535642678408545No Hit
ATGTCATAGATCATCTGGTTCTTGTGGGGTGTGTGTGTCAGAATCACATG15840.11448908522931787No Hit
CCCTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCATC15820.11434452830352329No Hit
CCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAATTCCAGATGAGAT15650.11311579443426924No Hit
GGTTCCTCCCTTGACACAGGAGGATTACAATTCCAGATGAGATTTCGGTG15630.11297123750847464No Hit
CCTGTGAGGGTCTCAGTCTATCTTGCAGCTCCCCTGGGGCTGGGGCTGAG15590.11268212365688546No Hit
CTTTAATTCAGCCAACATTTCGTGGGTCGCTCTATGGAGGTCACTCACTG15590.11268212365688546No Hit
AAAGCAGGGAGCCTTAGCTAAATGCTGTATGTCATAGATCATCTGGTTCT15440.11159794671342602No Hit
GTCTGAAAAGCCTTCAGATTCTTGACTCTTTAATTCAGCCAACATTTCGT15380.11116427593604224No Hit
AGCGAAAGCAGGGCCTGCAGGGCCATGAGTGGGAGCCCTCCTCCAGTGTT15210.10993554206678818No Hit
GAGTACAGTTCTGCTGGCCCTGCTCTACAGCACGTGGGGACTCTGCCTGT15170.109646428215199No Hit
TTCCAGATGAGATTTCGGTGGGGACACAGAGCCCAACCTGTGAGGGTCTC15130.10935731436360982No Hit
GAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGGAGCTGGATCTACAAC15070.10892364358622603No Hit
ATCAGGAGGCGGTGATAATGCTGGTGGGGGCACACAGGCAGAGTCCCCAC14740.10653845431061525No Hit
CCTCCAGAAGGGGGGCTGTGGGGCCTCCAGGATAAGGAGGGGGCGGCTCA14710.10632161892192336No Hit
GTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAAT14290.10328592348023691No Hit
CTCTGAAGGTGTTTCCCATGTGATTCTGACACACACACCCCACAAGAACC14280.10321364501733961No Hit
GCCATGAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGGAGCTGGATCT14250.10299680962864771No Hit
AGATAGACTGAGACCCTCACAGGTTGGGCTCTGTGTCCCCACCGAAATCT14120.10205718961098285No Hit
GGAGGGGGCGGCTCATTAGACATCTTTGCTGCTTCTGGACCTCAAATCCC14090.10184035422229097No Hit
CACCAGGTTCCTCCCTTGACACAGGAGGATTACAATTCCAGATGAGATTT13940.10075617727883153No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)